Determination of anti-staphylococcal activity of thymoquinone in combinations with antibiotics by checkerboard method using EVA capmatTM as a vapor barrier

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F16%3A67295" target="_blank" >RIV/60460709:41340/16:67295 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/17:73968 RIV/60460709:41340/17:73968 RIV/75010330:_____/17:00011772

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.arabjc.2015.04.021" target="_blank" >http://dx.doi.org/10.1016/j.arabjc.2015.04.021</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.arabjc.2015.04.021" target="_blank" >10.1016/j.arabjc.2015.04.021</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of anti-staphylococcal activity of thymoquinone in combinations with antibiotics by checkerboard method using EVA capmatTM as a vapor barrier

Popis výsledku v původním jazyce

Thymoquinone (Tq) has been reported to potentiate the in vitro growth-inhibitory activity of some antibiotics especially against Staphylococcus aureus. However, it has been shown that Tq vapors can affect the results of susceptibility testing by standard broth microdilution method. Therefore, we made a comparative experiment with and without ethylene vinyl acetate cap mats (EVA capmatTM) on microplates. The results showed significant differences in the minimum inhibitory concentration values and proved this capmat as an effective vapor barrier. Therefore further experiments focused on the in vitro anti-staphylococcal combinatory effect of Tq with oxacillin, penicillin, and tetracycline against various S. aureus strains have been performed by checkerboard method using EVA capmatTM. The combined effect was evaluated according to the sum of fractional inhibitory concentrations (?FIC). Synergy was obtained for combination with oxacillin against 3 (?FIC 0,263-0,450), with penicillin against 1 (?FIC 0,466)

Název v anglickém jazyce

Determination of anti-staphylococcal activity of thymoquinone in combinations with antibiotics by checkerboard method using EVA capmatTM as a vapor barrier

Popis výsledku anglicky

Thymoquinone (Tq) has been reported to potentiate the in vitro growth-inhibitory activity of some antibiotics especially against Staphylococcus aureus. However, it has been shown that Tq vapors can affect the results of susceptibility testing by standard broth microdilution method. Therefore, we made a comparative experiment with and without ethylene vinyl acetate cap mats (EVA capmatTM) on microplates. The results showed significant differences in the minimum inhibitory concentration values and proved this capmat as an effective vapor barrier. Therefore further experiments focused on the in vitro anti-staphylococcal combinatory effect of Tq with oxacillin, penicillin, and tetracycline against various S. aureus strains have been performed by checkerboard method using EVA capmatTM. The combined effect was evaluated according to the sum of fractional inhibitory concentrations (?FIC). Synergy was obtained for combination with oxacillin against 3 (?FIC 0,263-0,450), with penicillin against 1 (?FIC 0,466)

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.30.0040" target="_blank" >EE2.3.30.0040: Rozvoj vědeckých týmů na České zemědělské univerzitě v Praze s cílem posílení pedagogických aktivit v mimopražských pracovištích</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Arabian Journal of Chemistry

ISSN

1878-5352

e-ISSN

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Svazek periodika

N

Číslo periodika v rámci svazku

N

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

1-6

Kód UT WoS článku

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EID výsledku v databázi Scopus

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