A thaumatin-like genomic sequence identification in Vitis vinifera L., Stormy wines and musts based on direct PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F18%3A76468" target="_blank" >RIV/60460709:41340/18:76468 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.5219/892" target="_blank" >http://dx.doi.org/10.5219/892</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5219/892" target="_blank" >10.5219/892</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A thaumatin-like genomic sequence identification in Vitis vinifera L., Stormy wines and musts based on direct PCR

Popis výsledku v původním jazyce

Direct polymerase chain reaction method was use to amplify a thaumatin - like sequence of Vitis vinifera L. in grapes as well as in stormy wines and musts. Thaumatin-like proteins (TLPs) of Vitis vinifera possess beside its function in abiotic and biotic stress response another one - they are able to cause protein haze in wine unless removed prior to bottling. Direct PCR is an approach where omission of DNA extraction is typical prior the amplification of the target site of plant genome. Crude extract or small pieces of plant tissues are used in the analysis directly without steps of extraction and purification of gDNA. The biological material that was used in analysis was collected during August - October 2017 in local stores and winery Sabo and comprises from cultivars Irsai, Muskát, Savignon Blanc, Svatovavrinecké, Dornfelder and Pálava. Direct PCR was performed by a cutted piece of grape tissue and a dilution buffer was use in 1:2 for stormy wine or must, respectively. Direct amplification of tha

Název v anglickém jazyce

A thaumatin-like genomic sequence identification in Vitis vinifera L., Stormy wines and musts based on direct PCR

Popis výsledku anglicky

Direct polymerase chain reaction method was use to amplify a thaumatin - like sequence of Vitis vinifera L. in grapes as well as in stormy wines and musts. Thaumatin-like proteins (TLPs) of Vitis vinifera possess beside its function in abiotic and biotic stress response another one - they are able to cause protein haze in wine unless removed prior to bottling. Direct PCR is an approach where omission of DNA extraction is typical prior the amplification of the target site of plant genome. Crude extract or small pieces of plant tissues are used in the analysis directly without steps of extraction and purification of gDNA. The biological material that was used in analysis was collected during August - October 2017 in local stores and winery Sabo and comprises from cultivars Irsai, Muskát, Savignon Blanc, Svatovavrinecké, Dornfelder and Pálava. Direct PCR was performed by a cutted piece of grape tissue and a dilution buffer was use in 1:2 for stormy wine or must, respectively. Direct amplification of tha

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

21101 - Food and beverages

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Potravinárstvo

ISSN

1337-0960

e-ISSN

1337-0960

Svazek periodika

12

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

226-232

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85043466617