Working more effectively with Bacillus amyloliquefaciens alfa-amylase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F11%3A43893010" target="_blank" >RIV/60461373:22310/11:43893010 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Working more effectively with Bacillus amyloliquefaciens alfa-amylase

Popis výsledku v původním jazyce

alfa-Amylases (EC 3.2.1.1) are enzymes commonly used in paper conservation to remove of starch-based adhesives. However, the effectiveness of their use can be influenced by a number of factors. Some of the most important ones were studied in this work. At first, the optimal range of pH, temperature and concentration of calcium ions in the enzyme solution was verified. Next, the problem of an accessibility of the adhesive for the enzyme was investigated. In typical situations, enzyme molecules have to penetrate the complex porous structure of paper in order to reach the adhesive layer. Several possibilities how to increase penetration rate of the enzyme solution through the paper were tested. All experiments were performed using Bacillus amyloliquefaciens alfa-amylase (Sigma-Aldrich, A 6380, type II A).

Název v anglickém jazyce

Working more effectively with Bacillus amyloliquefaciens alfa-amylase

Popis výsledku anglicky

alfa-Amylases (EC 3.2.1.1) are enzymes commonly used in paper conservation to remove of starch-based adhesives. However, the effectiveness of their use can be influenced by a number of factors. Some of the most important ones were studied in this work. At first, the optimal range of pH, temperature and concentration of calcium ions in the enzyme solution was verified. Next, the problem of an accessibility of the adhesive for the enzyme was investigated. In typical situations, enzyme molecules have to penetrate the complex porous structure of paper in order to reach the adhesive layer. Several possibilities how to increase penetration rate of the enzyme solution through the paper were tested. All experiments were performed using Bacillus amyloliquefaciens alfa-amylase (Sigma-Aldrich, A 6380, type II A).

Druh

D - Stať ve sborníku

CEP obor

JJ - Ostatní materiály

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

New Approaches to Book and Paper Conservation-Restoration

ISBN

978-3-85028-518-6

ISSN

2225-7853

e-ISSN

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Počet stran výsledku

4

Strana od-do

695-698

Název nakladatele

Verlang Berger Horn

Místo vydání

Vídeň

Místo konání akce

Horn

Datum konání akce

9. 5. 2011

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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