Synthesis of magnetic microparticles for isolation of DNA from PCR products

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Synthesis of magnetic microparticles for isolation of DNA from PCR products

Popis výsledku v původním jazyce

Nowadays, analysis of DNA plays an important role in molecular biology and diagnostics (such as diagnosis of microbial infections, forensic analysis and molecular genetics). With the rapid development of analytical methods such as NGS (Next Generation Sequencing) and DNA microarray followed by their use in various research and clinical assays there is a need for techniques for fast isolation of DNA from tissue samples and PCR products. Currently, isolation on a solid phase is preferred due to easier manipulation, short separation times and lower cost. Presented work was focused on synthesis of superparamagnetic nanoparticles (Fe3O4), their encapsulation by silica shell and further functionalization with amino groups. Different precursors, solvents and their ratios were used for synthesis of silica shell to obtain layers of various thickness. Those particles were then used for DNA isolation from PCR products. Optimization of the isolation was carried out and subsequently the influence of magnetic particles synthesis on the DNA isolation was studied.

Název v anglickém jazyce

Synthesis of magnetic microparticles for isolation of DNA from PCR products

Popis výsledku anglicky

Nowadays, analysis of DNA plays an important role in molecular biology and diagnostics (such as diagnosis of microbial infections, forensic analysis and molecular genetics). With the rapid development of analytical methods such as NGS (Next Generation Sequencing) and DNA microarray followed by their use in various research and clinical assays there is a need for techniques for fast isolation of DNA from tissue samples and PCR products. Currently, isolation on a solid phase is preferred due to easier manipulation, short separation times and lower cost. Presented work was focused on synthesis of superparamagnetic nanoparticles (Fe3O4), their encapsulation by silica shell and further functionalization with amino groups. Different precursors, solvents and their ratios were used for synthesis of silica shell to obtain layers of various thickness. Those particles were then used for DNA isolation from PCR products. Optimization of the isolation was carried out and subsequently the influence of magnetic particles synthesis on the DNA isolation was studied.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 5th International Conference on Chemical Technology

ISBN

978-80-86238-65-4

ISSN

2336-811X

e-ISSN

neuvedeno

Počet stran výsledku

6

Strana od-do

292-297

Název nakladatele

Česká společnost průmyslové chemie (ČSPCH)

Místo vydání

Praha

Místo konání akce

Mikulov

Datum konání akce

10. 4. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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