Coating of magnetic nanoparticles by silica layer and further functionalization for isolation of DNA from PCR products

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F18%3A43917597" target="_blank" >RIV/60461373:22310/18:43917597 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.icct.cz/AngiologyKlon-ICCT/media/system/2018/ICCT-2018-Proceedings.pdf" target="_blank" >https://www.icct.cz/AngiologyKlon-ICCT/media/system/2018/ICCT-2018-Proceedings.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Coating of magnetic nanoparticles by silica layer and further functionalization for isolation of DNA from PCR products

Popis výsledku v původním jazyce

Nowadays, analysis of DNA plays an important role in molecular biology and diagnostics (such as diagnosis of microbial infections, forensic analysis and molecular genetics). With the rapid development of analytical methods such as NGS (Next Generation Sequencing) and DNA microarray followed by their use in various research and clinical assays there is a need for techniques for fast isolation of DNA from tissue samples and PCR products. Currently, isolation on a solid phase is preferred due to easier manipulation, short separation times and lower cost. Presented work was focused on synthesis of superparamagnetic nanoparticles (Fe3O4), their encapsulation by silica shell and further functionalization with amino groups. Different precursors, solvents and their ratios were used for synthesis of silica shell to obtain layers of various thickness. Those particles were then used for DNA isolation from PCR products. Optimization of the isolation was carried out and subsequently the influence of magnetic particles synthesis on the DNA isolation was studied.

Název v anglickém jazyce

Coating of magnetic nanoparticles by silica layer and further functionalization for isolation of DNA from PCR products

Popis výsledku anglicky

Nowadays, analysis of DNA plays an important role in molecular biology and diagnostics (such as diagnosis of microbial infections, forensic analysis and molecular genetics). With the rapid development of analytical methods such as NGS (Next Generation Sequencing) and DNA microarray followed by their use in various research and clinical assays there is a need for techniques for fast isolation of DNA from tissue samples and PCR products. Currently, isolation on a solid phase is preferred due to easier manipulation, short separation times and lower cost. Presented work was focused on synthesis of superparamagnetic nanoparticles (Fe3O4), their encapsulation by silica shell and further functionalization with amino groups. Different precursors, solvents and their ratios were used for synthesis of silica shell to obtain layers of various thickness. Those particles were then used for DNA isolation from PCR products. Optimization of the isolation was carried out and subsequently the influence of magnetic particles synthesis on the DNA isolation was studied.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

PROCEEDINGS of the 6th International Conference on Chemical Technology

ISBN

978-80-86238-77-7

ISSN

2336-8128

e-ISSN

neuvedeno

Počet stran výsledku

6

Strana od-do

369-374

Název nakladatele

Česká společnost průmyslové chemie (ČSPCH)

Místo vydání

Praha

Místo konání akce

Mikulov

Datum konání akce

16. 4. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000461293800068