Thiographene synthesized from fluorographene via xanthogenate with immobilized enzymes for environmental remediation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F19%3A43918018" target="_blank" >RIV/60461373:22310/19:43918018 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/19:PU133202

Výsledek na webu

<a href="https://pubs.rsc.org/en/content/articlepdf/2019/nr/c9nr02376c" target="_blank" >https://pubs.rsc.org/en/content/articlepdf/2019/nr/c9nr02376c</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c9nr02376c" target="_blank" >10.1039/c9nr02376c</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Thiographene synthesized from fluorographene via xanthogenate with immobilized enzymes for environmental remediation

Popis výsledku v původním jazyce

Graphene, graphene oxide and their related thiographene-, hydroxygraphene- or fluorographene-based materials have broad applications. We report on the thiol-functionalization of fluorographene via xanthogenate. Such thiographene contains 5.1 at% of sulphur in the form of thiol groups, which is the highest thiol content reported to date. Such tailored thiographene allows the immobilization of two types of enzymes. Here, we explore the functionalization of highly thiolated graphene with enzymes via physisorption or covalent linkage producing an important heterogeneous biocatalyst platform for wastewater treatment applications. Thiographene modified with a lipase from Mucor miehei can find utilization in lipidrich wastewater treatment whereas the catalase-modified thiographene is intended for bioremediation applications. Upon increasing concentration of the thiol groups on graphene, protein loading of the catalase was increased by 16% and the ester bond cleavage activity of the thiographene-immobilized lipase was 129% that of the free lipase. We expect that such a highly active heterogeneous thiographene-based biocatalyst will find a use in water remediation applications.

Název v anglickém jazyce

Thiographene synthesized from fluorographene via xanthogenate with immobilized enzymes for environmental remediation

Popis výsledku anglicky

Graphene, graphene oxide and their related thiographene-, hydroxygraphene- or fluorographene-based materials have broad applications. We report on the thiol-functionalization of fluorographene via xanthogenate. Such thiographene contains 5.1 at% of sulphur in the form of thiol groups, which is the highest thiol content reported to date. Such tailored thiographene allows the immobilization of two types of enzymes. Here, we explore the functionalization of highly thiolated graphene with enzymes via physisorption or covalent linkage producing an important heterogeneous biocatalyst platform for wastewater treatment applications. Thiographene modified with a lipase from Mucor miehei can find utilization in lipidrich wastewater treatment whereas the catalase-modified thiographene is intended for bioremediation applications. Upon increasing concentration of the thiol groups on graphene, protein loading of the catalase was increased by 16% and the ester bond cleavage activity of the thiographene-immobilized lipase was 129% that of the free lipase. We expect that such a highly active heterogeneous thiographene-based biocatalyst will find a use in water remediation applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

<a href="/cs/project/GA17-05421S" target="_blank" >GA17-05421S: Nové účinné membrány pro efektivní separace H2 / CO2 (HySME)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nanoscale

ISSN

2040-3364

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

22

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

10695-10701

Kód UT WoS článku

000470756000010

EID výsledku v databázi Scopus

2-s2.0-85067010857