Enzymatic Determination of Anticholinesterases Using a Composite Carrier

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F16%3A43902414" target="_blank" >RIV/60461373:22320/16:43902414 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/45808121:_____/16:N0000001 RIV/62157124:16370/16:43874419

Výsledek na webu

<a href="http://dx.doi.org/10.1080/00032719.2016.1151889" target="_blank" >http://dx.doi.org/10.1080/00032719.2016.1151889</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/00032719.2016.1151889" target="_blank" >10.1080/00032719.2016.1151889</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enzymatic Determination of Anticholinesterases Using a Composite Carrier

Popis výsledku v původním jazyce

A modified enzyme method is reported for the determination of cholinesterase inhibitors based on the use of a granulated composite carrier with immobilized butyrylcholinesterase. The detection system includes butyrylthiocholine as the substrate and N-(2,3-dimethyl-5-oxo-1-phenyl-3-pyrazoline-4-yl)-2-chloro-5-sulpho-4-iminobenzoquinone as a redox indicator. This method was characterized using carbamate physostigmine and sarin (O-isopropyl methylphosphonofluoridate). The method was also used in a simple tube detector. Evaluation was based on the observation with the naked eye of the decolorization of the redox indicator in comparison to a control time and by tristimulus colorimetry. The limits of detection were 0.5 ?g/mL for physostigmine and 7 x 10?4 mg/m3 for sarin visually, or 0.06 ?g/mL for physostigmine and 1.1 x 10?4 mg/m3 for sarin by colorimetry. ? 2016, Copyright ? Taylor & Francis Group, LLC.

Název v anglickém jazyce

Enzymatic Determination of Anticholinesterases Using a Composite Carrier

Popis výsledku anglicky

A modified enzyme method is reported for the determination of cholinesterase inhibitors based on the use of a granulated composite carrier with immobilized butyrylcholinesterase. The detection system includes butyrylthiocholine as the substrate and N-(2,3-dimethyl-5-oxo-1-phenyl-3-pyrazoline-4-yl)-2-chloro-5-sulpho-4-iminobenzoquinone as a redox indicator. This method was characterized using carbamate physostigmine and sarin (O-isopropyl methylphosphonofluoridate). The method was also used in a simple tube detector. Evaluation was based on the observation with the naked eye of the decolorization of the redox indicator in comparison to a control time and by tristimulus colorimetry. The limits of detection were 0.5 ?g/mL for physostigmine and 7 x 10?4 mg/m3 for sarin visually, or 0.06 ?g/mL for physostigmine and 1.1 x 10?4 mg/m3 for sarin by colorimetry. ? 2016, Copyright ? Taylor & Francis Group, LLC.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/TA04011060" target="_blank" >TA04011060: Technologie pro nový kolorimetrický trubičkový biosenzor inhibitorů cholinesteráz v ovzduší i v kapalných vzorcích</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ANALYTICAL LETTERS

ISSN

0003-2719

e-ISSN

—

Svazek periodika

49

Číslo periodika v rámci svazku

15

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

2418-2426

Kód UT WoS článku

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EID výsledku v databázi Scopus

2-s2.0-84986296785