Biochemical Detection of Cholinesterase Inhibitors in Water with a New Two-Component Chromogenic Reagent

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F18%3A43915528" target="_blank" >RIV/60461373:22320/18:43915528 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16370/18:43876651

Výsledek na webu

<a href="https://link.springer.com/article/10.1134/S1061934818090101" target="_blank" >https://link.springer.com/article/10.1134/S1061934818090101</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1134/S1061934818090101" target="_blank" >10.1134/S1061934818090101</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biochemical Detection of Cholinesterase Inhibitors in Water with a New Two-Component Chromogenic Reagent

Popis výsledku v původním jazyce

We propose a simple biochemical detector of cholinesterase inhibitors in water that is based on an enzymatic reaction. The detector was designed as a detection tube containing (i) an indication layer with an immobilized butyrylcholinesterase, (ii) a layer with substrate and chromogenic reagent and (iii) an ampoule with buffer. The chromogenic reagent contained a mixture of two triphenylmethane dyes: Guinea Green B and Fuschsin Basic. The absence of inhibitors was indicated by changing the blue color of the indication layer to violet-red. In the presence of inhibitors, the indication layer remained blue-colored. Color change was evaluated visually (by naked eye). The functionality of the detector was verified on aqueous solutions of alkaloid physostigmine and two chemical warfare agents: sarin and VX. The achieved detection limits were 0.01 μg/mL for physostigmine and sarin and 0.005 μg/mL for VX.

Název v anglickém jazyce

Biochemical Detection of Cholinesterase Inhibitors in Water with a New Two-Component Chromogenic Reagent

Popis výsledku anglicky

We propose a simple biochemical detector of cholinesterase inhibitors in water that is based on an enzymatic reaction. The detector was designed as a detection tube containing (i) an indication layer with an immobilized butyrylcholinesterase, (ii) a layer with substrate and chromogenic reagent and (iii) an ampoule with buffer. The chromogenic reagent contained a mixture of two triphenylmethane dyes: Guinea Green B and Fuschsin Basic. The absence of inhibitors was indicated by changing the blue color of the indication layer to violet-red. In the presence of inhibitors, the indication layer remained blue-colored. Color change was evaluated visually (by naked eye). The functionality of the detector was verified on aqueous solutions of alkaloid physostigmine and two chemical warfare agents: sarin and VX. The achieved detection limits were 0.01 μg/mL for physostigmine and sarin and 0.005 μg/mL for VX.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/TA04011060" target="_blank" >TA04011060: Technologie pro nový kolorimetrický trubičkový biosenzor inhibitorů cholinesteráz v ovzduší i v kapalných vzorcích</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF ANALYTICAL CHEMISTRY

ISSN

1061-9348

e-ISSN

—

Svazek periodika

73

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

RU - Ruská federace

Počet stran výsledku

6

Strana od-do

900-905

Kód UT WoS článku

000443826400009

EID výsledku v databázi Scopus

2-s2.0-85052890296