Analysis of aldehydes in beer using SPME with on-fiber derivatization and Gas Chromatography/Mass Spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008548" target="_blank" >RIV/60461373:22330/03:00008548 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of aldehydes in beer using SPME with on-fiber derivatization and Gas Chromatography/Mass Spectrometry

Popis výsledku v původním jazyce

A new, fast, sensitive and solventless extraction technique was developed in order to analyze beer carbonyl compounds. The method was based on solid-phase microextraction (SPME) with on-fiber derivatization. A derivatization agent, O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine (PFBOA), was absorbed onto a divinylbenzene/poly(dimethylsiloxane) (DVB/PDMS) 65 m fiber and exposed to the headspace of a vial with a beer sample. Carbonyl compounds selectively reacted with PFBOA and the oximes formed were desorbed into a gas chromatograph injection port and quantified by mass spectrometry. This method provided very high reproducibility and linearity. When used for the analysis of aged beers, nine aldehydes were detected: 2-methylpropanal, 2-methylbutanal, 3-methylbutanal, pentanal, hexanal, furfural, methional, phenylacetaldehyde and (E)-2-nonenal.

Název v anglickém jazyce

Analysis of aldehydes in beer using SPME with on-fiber derivatization and Gas Chromatography/Mass Spectrometry

Popis výsledku anglicky

A new, fast, sensitive and solventless extraction technique was developed in order to analyze beer carbonyl compounds. The method was based on solid-phase microextraction (SPME) with on-fiber derivatization. A derivatization agent, O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine (PFBOA), was absorbed onto a divinylbenzene/poly(dimethylsiloxane) (DVB/PDMS) 65 m fiber and exposed to the headspace of a vial with a beer sample. Carbonyl compounds selectively reacted with PFBOA and the oximes formed were desorbed into a gas chromatograph injection port and quantified by mass spectrometry. This method provided very high reproducibility and linearity. When used for the analysis of aged beers, nine aldehydes were detected: 2-methylpropanal, 2-methylbutanal, 3-methylbutanal, pentanal, hexanal, furfural, methional, phenylacetaldehyde and (E)-2-nonenal.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Agricultural and Food Chemistry

ISSN

0021-5861

e-ISSN

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Svazek periodika

51

Číslo periodika v rámci svazku

24

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

6941-6944

Kód UT WoS článku

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EID výsledku v databázi Scopus

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