System for detection of GM potatoes and wheat in food - sborník

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008841" target="_blank" >RIV/60461373:22330/03:00008841 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

System for detection of GM potatoes and wheat in food - sborník

Popis výsledku v původním jazyce

The detection of genetically modified organisms is often pursued by the polymerase chain reaction (PCR). The detection includes basically three steps: DNA extraction from the sample, PCR with detection of products, and data evaluation. In the presented report a suitable protocol for the DNA isolation was searched for. From the published methods, five were chosen and used for the isolation of the DNA from wheat, soybean, potatoes and corn. The methods were evaluated by means of different parameters, likethe DNA purity, amplifiability by PCR, cost and duration of the procedure. The modified CTAB method and DNeasyTM Plant Mini Kit were found the most suitable methods of extraction. Then the selection of optimal conditions for detection of transgenic wheat and transgenic potato was performed. The samples were tested for possible occurrence of sequences of transgenic DNA that are used in commonly available genetically modified products. The most frequent sequences, regulating expression of

Název v anglickém jazyce

System for detection of GM potatoes and wheat in food - sborník

Popis výsledku anglicky

The detection of genetically modified organisms is often pursued by the polymerase chain reaction (PCR). The detection includes basically three steps: DNA extraction from the sample, PCR with detection of products, and data evaluation. In the presented report a suitable protocol for the DNA isolation was searched for. From the published methods, five were chosen and used for the isolation of the DNA from wheat, soybean, potatoes and corn. The methods were evaluated by means of different parameters, likethe DNA purity, amplifiability by PCR, cost and duration of the procedure. The modified CTAB method and DNeasyTM Plant Mini Kit were found the most suitable methods of extraction. Then the selection of optimal conditions for detection of transgenic wheat and transgenic potato was performed. The samples were tested for possible occurrence of sequences of transgenic DNA that are used in commonly available genetically modified products. The most frequent sequences, regulating expression of

Druh

D - Stať ve sborníku

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

First International Symposium on Recent Advances in Food Analysis

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

128

Název nakladatele

FIRMA -JK

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

5. 11. 2003

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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