Optimisation of enzyme-linked immunosorbent assay for the detection of Listeria monocytogenes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008921" target="_blank" >RIV/60461373:22330/03:00008921 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimisation of enzyme-linked immunosorbent assay for the detection of Listeria monocytogenes

Popis výsledku v původním jazyce

Listeria monocytogenes is a gram-positive rod-shaped bacterium associated with a variety of environments including soils, water, sewage, silage, as well as plant and animal food products. Although reported cases of human foodborne listeriosis are rare, the incidence of serious illness and death in affected individuals is high. The detection of Listeria monocytogenes cells by conventional microbiological methods takes 5-6 days. We present here optimizations of a rapid, reliable and specific antibody-based assay. A model system consists of an enrichment stage and end-point detection by enzyme linked immunosorbent assay. Listeria cells were cultivated in selective medium in first step. Enrichment stage was followed by direct sandwich format of ELISA. It was found both convenient culture medium for cultivation of bacterial cells for use in the ELISA and optimal buffers for a whole method. From all tested antibodies, which have been raised, was found as the best that one against Listeria mo

Název v anglickém jazyce

Optimisation of enzyme-linked immunosorbent assay for the detection of Listeria monocytogenes

Popis výsledku anglicky

Listeria monocytogenes is a gram-positive rod-shaped bacterium associated with a variety of environments including soils, water, sewage, silage, as well as plant and animal food products. Although reported cases of human foodborne listeriosis are rare, the incidence of serious illness and death in affected individuals is high. The detection of Listeria monocytogenes cells by conventional microbiological methods takes 5-6 days. We present here optimizations of a rapid, reliable and specific antibody-based assay. A model system consists of an enrichment stage and end-point detection by enzyme linked immunosorbent assay. Listeria cells were cultivated in selective medium in first step. Enrichment stage was followed by direct sandwich format of ELISA. It was found both convenient culture medium for cultivation of bacterial cells for use in the ELISA and optimal buffers for a whole method. From all tested antibodies, which have been raised, was found as the best that one against Listeria mo

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F03%2F0350" target="_blank" >GA525/03/0350: Vývoj screeningové imunochemické metody pro specifickou detekci Listeria monocytogenes v potravinách</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

1st International Symposium on Recent Advances in Food Analysis, Book of Abstracts

ISBN

80-7080-528-5

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

39

Název nakladatele

VŠCHT

Místo vydání

FIRMA-JK

Místo konání akce

CZ

Datum konání akce

5. 11. 2003

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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