Vliv podmínek assembly na morfologii částic tvořených z CA-NC proteinu MPMV

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012908" target="_blank" >RIV/60461373:22330/04:00012908 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The influence of assembly conditions on effeiciency and morphology of in vitro assembled capsid-like particles formed from CA-NC of Mason-Pfirzer money virus

Popis výsledku v původním jazyce

Domains within structural polyprotein Gag of all retroviruses initiate and direct assembly of the immature viral particles. Gag forms capsid-like particles in bacteria, mammalian cells and in vitro. Several studies have shown that Gag and its deletion mutants, e.g. capsid-nucleocapsid fusion protein (CA-NC) or CA assemble in vitro into spherical, conical or tubular particles. Morphology of formed particles depends on virus type and assembly conditions. CA itself can assemble in vitro into tubular or conical structures resembling a mature core. NC facilitates Gag interactions and it is crucial for specific incorporation of genomic RNA. In previous study we have shown that Mason-Pfizer monkey virus deletion construct consisting of CA and NC fusion protein that lacks the N-terminal proline (Pro-)CA-NC assembles into spherical particles when expressed in E. coli in contrast with tubular structures formed from CA-NC under the same conditions. To evaluate the effect of nucleic acids on the a

Název v anglickém jazyce

The influence of assembly conditions on effeiciency and morphology of in vitro assembled capsid-like particles formed from CA-NC of Mason-Pfirzer money virus

Popis výsledku anglicky

Domains within structural polyprotein Gag of all retroviruses initiate and direct assembly of the immature viral particles. Gag forms capsid-like particles in bacteria, mammalian cells and in vitro. Several studies have shown that Gag and its deletion mutants, e.g. capsid-nucleocapsid fusion protein (CA-NC) or CA assemble in vitro into spherical, conical or tubular particles. Morphology of formed particles depends on virus type and assembly conditions. CA itself can assemble in vitro into tubular or conical structures resembling a mature core. NC facilitates Gag interactions and it is crucial for specific incorporation of genomic RNA. In previous study we have shown that Mason-Pfizer monkey virus deletion construct consisting of CA and NC fusion protein that lacks the N-terminal proline (Pro-)CA-NC assembles into spherical particles when expressed in E. coli in contrast with tubular structures formed from CA-NC under the same conditions. To evaluate the effect of nucleic acids on the a

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/LN00A079" target="_blank" >LN00A079: Centrum integrované genomiky</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Virus Assembly Conference

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

254

Název nakladatele

Vermont Academy

Místo vydání

Vermont

Místo konání akce

Vermont

Datum konání akce

1. 1. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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