Záhadná role nukleových kyselin v assembly Mason-Pfizerova opičího viru

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00015669" target="_blank" >RIV/60461373:22330/05:00015669 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/05:00016396

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The puzzling role of nucleic acids in the assembly of Mason-Pfizer monkey virus

Popis výsledku v původním jazyce

All retroviruses form a structural polyprotein precursor Gag that directs the assembly of the immature retroviral capsid. The role of nucleic acid in the assembly process was extensively studied and several models predicted how the nucleic acid could work and participate on retroviral capsid assembly, but still the exact function of nucleic acid is not clear. We have studied the in vitro assembly process of Mason-Pfizer monkey virus Gag deletion mutant, consisting of two structural proteins, capsid andnucleocapsid. We have focused mainly on (pro-)CANC, CA-NC fusion protein lacking its N-terminal proline, aminoacid that is determining the shape of assembled particles. This mutant assembles with high efficiency in vitro, forming spherical particles of 75 nm diameter in average.To study the role of nucleic acids in the assembly process we optimized the protein purification method that provides the nucleic acid free protein. For studying the assembly process we have used several nucleic a

Název v anglickém jazyce

The puzzling role of nucleic acids in the assembly of Mason-Pfizer monkey virus

Popis výsledku anglicky

All retroviruses form a structural polyprotein precursor Gag that directs the assembly of the immature retroviral capsid. The role of nucleic acid in the assembly process was extensively studied and several models predicted how the nucleic acid could work and participate on retroviral capsid assembly, but still the exact function of nucleic acid is not clear. We have studied the in vitro assembly process of Mason-Pfizer monkey virus Gag deletion mutant, consisting of two structural proteins, capsid andnucleocapsid. We have focused mainly on (pro-)CANC, CA-NC fusion protein lacking its N-terminal proline, aminoacid that is determining the shape of assembled particles. This mutant assembles with high efficiency in vitro, forming spherical particles of 75 nm diameter in average.To study the role of nucleic acids in the assembly process we optimized the protein purification method that provides the nucleic acid free protein. For studying the assembly process we have used several nucleic a

Druh

O - Ostatní výsledky

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů