Studium tvorby kapsid retrovirů jako systému k přenosu nukleových kyselin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00015671" target="_blank" >RIV/60461373:22330/05:00015671 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The study of formation of retroviral capsids as a nucleic acids delivery system

Popis výsledku v původním jazyce

Our research is focused on study of formation of Mason-Pfizer monkey virus (M-PMV) capsids in vitro. The understanding of retroviral capsid assembly mechanism and nucleic acids incorporation could significantly contribute to further use of these systemsfor therapeutic purposes. All retroviruses form a single structural precursor polyprotein Gag that initiates and directs the assembly of immature retroviral capsid. Our interest has been focused on minimized Gag domain, namely fused capsid and nucleocapsid proteins (CA-NC), which contain domains responsible for interactions during capsid assembly process. CA-NC protein was purified by several techniques to ensure the elimination of nucleic acids that could contaminate final product. We optimized the assembly reaction by monitoring of different conditions as various concentrations of salts, pH, temperature and presence of oligonucleotides or RNA. The assembly reaction of protein and RNA or oligonucleotide were performed during dialysis a

Název v anglickém jazyce

The study of formation of retroviral capsids as a nucleic acids delivery system

Popis výsledku anglicky

Our research is focused on study of formation of Mason-Pfizer monkey virus (M-PMV) capsids in vitro. The understanding of retroviral capsid assembly mechanism and nucleic acids incorporation could significantly contribute to further use of these systemsfor therapeutic purposes. All retroviruses form a single structural precursor polyprotein Gag that initiates and directs the assembly of immature retroviral capsid. Our interest has been focused on minimized Gag domain, namely fused capsid and nucleocapsid proteins (CA-NC), which contain domains responsible for interactions during capsid assembly process. CA-NC protein was purified by several techniques to ensure the elimination of nucleic acids that could contaminate final product. We optimized the assembly reaction by monitoring of different conditions as various concentrations of salts, pH, temperature and presence of oligonucleotides or RNA. The assembly reaction of protein and RNA or oligonucleotide were performed during dialysis a

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/1M0520" target="_blank" >1M0520: Centrum aplikované genomiky</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů