Preparace a in vitro fermentace N-alkylamidovaných pektinů pomoci kultur střevné mikroflory prasat

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00013004" target="_blank" >RIV/60461373:22330/04:00013004 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Preparation and in vitro fermentation of N-alkylamidated pectins by pig colonic microflora cultures

Popis výsledku v původním jazyce

N-Alkylamides of highly methylated (HM) lime peel pectin (type XSS, Danisco) were prepared using heterogeneous amino-de-alkoxylation (aminolysis) with five chosen n-alkylamines (n-butylamine, n-hexylamine, n-octylamine, n-dodecylamine and n-octadecylamine). The experiment was carried out in N,N-dimethylformamide at 50°C for 3-7 days. The diffusion reflection FT-IR spectra of the products indicate the presence of alkylamide substituents bound to pectin. The degrees of amidation (DA) of the derivatives were calculated based on Kjeldahl nitrogen. N-Alkylpectinamides of low (DA = 5-10 %) and high (DA = 15-40 %) substitution degrees were used in the in vitro experiments on digestion by pig colonic microflora cultures. Production of volatile fatty acids as well as fermentation gas was determined in these cultures. Highly methylated lime peel pectin that was used for syntheses of amidated derivatives served as control. It was found that the fermentation rate of low substituted derivatives was

Název v anglickém jazyce

Preparation and in vitro fermentation of N-alkylamidated pectins by pig colonic microflora cultures

Popis výsledku anglicky

N-Alkylamides of highly methylated (HM) lime peel pectin (type XSS, Danisco) were prepared using heterogeneous amino-de-alkoxylation (aminolysis) with five chosen n-alkylamines (n-butylamine, n-hexylamine, n-octylamine, n-dodecylamine and n-octadecylamine). The experiment was carried out in N,N-dimethylformamide at 50°C for 3-7 days. The diffusion reflection FT-IR spectra of the products indicate the presence of alkylamide substituents bound to pectin. The degrees of amidation (DA) of the derivatives were calculated based on Kjeldahl nitrogen. N-Alkylpectinamides of low (DA = 5-10 %) and high (DA = 15-40 %) substitution degrees were used in the in vitro experiments on digestion by pig colonic microflora cultures. Production of volatile fatty acids as well as fermentation gas was determined in these cultures. Highly methylated lime peel pectin that was used for syntheses of amidated derivatives served as control. It was found that the fermentation rate of low substituted derivatives was

Druh

D - Stať ve sborníku

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F03%2F0358" target="_blank" >GA525/03/0358: Příprava, popis struktury a biologické účinky derivátů pektinu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

CUKRBLIK 2004

ISBN

80-7080-536-6

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

33

Název nakladatele

Česká chemická společnost

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

1. 1. 2004

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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