Analýza Saccharomyces cerevisiae průtokovou cytometrií

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F06%3A00017747" target="_blank" >RIV/60461373:22330/06:00017747 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Flow cytometric analysis of Saccharomyces cerevisiae

Popis výsledku v původním jazyce

Two methods of preservation and storage of yeast cultures are tested. Freest yeast population suspended at medium containing glycerol (5% v/v) as a cryoprotectant and periodic sub-culture using agar slopes (slants). This work was carried out with three widely used Czech lager strains - No. 2, 7 and 95. All yeasts were analyzed by flow cytometry prior to storage and after storage. Fluorescent intensity of DNA was analysed by flow cytometry and signal was used as a marker for cell cycle determination. During experiments were monitored changes in significant properties of yeast (glycogen, proteins, DNA) by fluorescent methods. It seems that the impact of storage conditions has not influence on basic characteristic of yeast population, dynamic of microbialgrowth, cell cycle and technological properties of yeast cultures.

Název v anglickém jazyce

Flow cytometric analysis of Saccharomyces cerevisiae

Popis výsledku anglicky

Two methods of preservation and storage of yeast cultures are tested. Freest yeast population suspended at medium containing glycerol (5% v/v) as a cryoprotectant and periodic sub-culture using agar slopes (slants). This work was carried out with three widely used Czech lager strains - No. 2, 7 and 95. All yeasts were analyzed by flow cytometry prior to storage and after storage. Fluorescent intensity of DNA was analysed by flow cytometry and signal was used as a marker for cell cycle determination. During experiments were monitored changes in significant properties of yeast (glycogen, proteins, DNA) by fluorescent methods. It seems that the impact of storage conditions has not influence on basic characteristic of yeast population, dynamic of microbialgrowth, cell cycle and technological properties of yeast cultures.

Druh

A - Audiovizuální tvorba

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/1M0570" target="_blank" >1M0570: Výzkumné centrum pro studium obsahových látek ječmene a chmele</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

ISBN

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Místo vydání

Zoeterwoude, The Netherlands

Název nakladatele resp. objednatele

European Brewery Convention

Verze

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Identifikační číslo nosiče

2006