Fysiologie mikroorganismů schopných degradovat methyl terc-butyl ether (MTBE)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F07%3A00018341" target="_blank" >RIV/60461373:22330/07:00018341 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Physiology of methyl tert-butyl ether (MTBE) degrading microorganisms

Popis výsledku v původním jazyce

Several bacterial strains and consortia capable to utilize MTBE were isolated from fuel-contaminated sites. The screening was based on growth monitoring of selected microorganisms in the presence of MTBE. The ability of microorganisms to degrade MTBE asthe sole carbon source or by cometabolism with other substrates was monitored by GC with FID detection. Enzymes responsible for MTBE utilization have not yet been defined. We tested our isolated bacterial consortia for endogenous respiration and for theability of the strains to aerobically metabolize MTBE by assessment of their respiration with this and other substrates on the OROBOROS Oxygraph-2k. The respiration rate of cultures in response to added MTBE and other substrates, as well as the effect ofvarious enzyme inhibitors on the respiration will be presented. Preliminary results allow us to predict the presence of responsible enzymes.

Název v anglickém jazyce

Physiology of methyl tert-butyl ether (MTBE) degrading microorganisms

Popis výsledku anglicky

Several bacterial strains and consortia capable to utilize MTBE were isolated from fuel-contaminated sites. The screening was based on growth monitoring of selected microorganisms in the presence of MTBE. The ability of microorganisms to degrade MTBE asthe sole carbon source or by cometabolism with other substrates was monitored by GC with FID detection. Enzymes responsible for MTBE utilization have not yet been defined. We tested our isolated bacterial consortia for endogenous respiration and for theability of the strains to aerobically metabolize MTBE by assessment of their respiration with this and other substrates on the OROBOROS Oxygraph-2k. The respiration rate of cultures in response to added MTBE and other substrates, as well as the effect ofvarious enzyme inhibitors on the respiration will be presented. Preliminary results allow us to predict the presence of responsible enzymes.

Druh

D - Stať ve sborníku

CEP obor

DK - Kontaminace a dekontaminace půdy včetně pesticidů

OECD FORD obor

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Projekt

<a href="/cs/project/GA104%2F05%2F2637" target="_blank" >GA104/05/2637: Technologie odstraňování methyl t-butyletheru (MTBE) z vody</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Book of Proceedings - 4th Symposium on Biosorption and Bioremediation

ISBN

978-80-7080-026-3

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

113-116

Název nakladatele

VŠCHT Praha

Místo vydání

Praha

Místo konání akce

Praha, ČR

Datum konání akce

26. 8. 2007

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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