Imunoafinitní sorbenty pro SPE isoflavonoidů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F08%3A00019963" target="_blank" >RIV/60461373:22330/08:00019963 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Immunoaffinity sorbents for SPE purification of isoflavonoids

Popis výsledku v původním jazyce

Immunoaffinity chromatography (IAC) or immunoextraction (IE) is based on a highly selective and affinitive interaction between antibodies immobilized on a solid support and analytes-antigens present in a sample. Thus enabling to concentrate and clean-upthe analytes from complex biological matrixes, such as plant extracts. Isoflavonoids belong to secondary plant metabolites and some of them are known for their pharmacological activities such as estrogenic, antiviral, antibacterial, antioxidative etc. Legumes are known as their main source. Nevertheless, the presence of isoflavonoids has been reported in about sixty other families. In non-legumes, isoflavonoids are present in lower concentrations, which results in a more difficult detection and identification. This was the reason for the development of an off-line combination of IAC and HPLC-MS for the detection of isoflavonoids in non-legume plant extracts. Immunosorbents were prepared by immobilization of polyclonal rabbit antibodies

Název v anglickém jazyce

Immunoaffinity sorbents for SPE purification of isoflavonoids

Popis výsledku anglicky

Immunoaffinity chromatography (IAC) or immunoextraction (IE) is based on a highly selective and affinitive interaction between antibodies immobilized on a solid support and analytes-antigens present in a sample. Thus enabling to concentrate and clean-upthe analytes from complex biological matrixes, such as plant extracts. Isoflavonoids belong to secondary plant metabolites and some of them are known for their pharmacological activities such as estrogenic, antiviral, antibacterial, antioxidative etc. Legumes are known as their main source. Nevertheless, the presence of isoflavonoids has been reported in about sixty other families. In non-legumes, isoflavonoids are present in lower concentrations, which results in a more difficult detection and identification. This was the reason for the development of an off-line combination of IAC and HPLC-MS for the detection of isoflavonoids in non-legume plant extracts. Immunosorbents were prepared by immobilization of polyclonal rabbit antibodies

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F06%2F0864" target="_blank" >GA525/06/0864: Revize taxonomické distribuce isoflavonoidů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Polyphenols Communications

ISBN

978-84-691-4333-9

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

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Název nakladatele

University of Salamanca

Místo vydání

Salamanca

Místo konání akce

Salamanca, Španělsko

Datum konání akce

8. 6. 2008

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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