Development of sorbents for immunoaffinity extraction of isoflavonoids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F11%3A43892098" target="_blank" >RIV/60461373:22330/11:43892098 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.phytol.2010.12.005" target="_blank" >http://dx.doi.org/10.1016/j.phytol.2010.12.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.phytol.2010.12.005" target="_blank" >10.1016/j.phytol.2010.12.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of sorbents for immunoaffinity extraction of isoflavonoids

Popis výsledku v původním jazyce

The majority of currently known isoflavonoids has been detected in and isolated from the Leguminosae; however, their presence has also been reported in numerous non-legumes. Detection of these secondary metabolites in non-legumes is impeded by the complex matrix of plant material and low concentration of the analytes. In this study, we present a combination of immunoaffinity chromatography (IAC) and HPLC-ESI-MS as a new tool for the detection of isoflavonoids. Immunoglobulin fractions of polyclonal rabbit antisera against conjugates of bovine serum albumin (BSA) with selected isoflavonoids (i.e. biochanin A, genistein and daidzein) were immobilized on a commercially available Affi-Gel 10 support. Immunosorbents were characterized according to their specificity and binding capacity and used in an off-line combination with HPLC-ESI-MS for a pilot study of isoflavonoid presence in Maclura pomifera and Ficus carica (Moraceae). (C) 2011 Phytochemical Society of Europe. Published by Elsevier

Název v anglickém jazyce

Development of sorbents for immunoaffinity extraction of isoflavonoids

Popis výsledku anglicky

The majority of currently known isoflavonoids has been detected in and isolated from the Leguminosae; however, their presence has also been reported in numerous non-legumes. Detection of these secondary metabolites in non-legumes is impeded by the complex matrix of plant material and low concentration of the analytes. In this study, we present a combination of immunoaffinity chromatography (IAC) and HPLC-ESI-MS as a new tool for the detection of isoflavonoids. Immunoglobulin fractions of polyclonal rabbit antisera against conjugates of bovine serum albumin (BSA) with selected isoflavonoids (i.e. biochanin A, genistein and daidzein) were immobilized on a commercially available Affi-Gel 10 support. Immunosorbents were characterized according to their specificity and binding capacity and used in an off-line combination with HPLC-ESI-MS for a pilot study of isoflavonoid presence in Maclura pomifera and Ficus carica (Moraceae). (C) 2011 Phytochemical Society of Europe. Published by Elsevier

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F09%2F0994" target="_blank" >GA525/09/0994: Chemotaxonomie isoflavonoidů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PHYTOCHEMISTRY LETTERS

ISSN

1874-3900

e-ISSN

—

Svazek periodika

4

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

113-117

Kód UT WoS článku

000290788100013

EID výsledku v databázi Scopus

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