Analysis of isoflavonoids in Ficus carica by HPLC-MS and a comparison of extract Cleanup by Immunoaffinity Chromatography (IAC) and Solid Phase Extraction (SPE)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F10%3A00024198" target="_blank" >RIV/60461373:22330/10:00024198 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of isoflavonoids in Ficus carica by HPLC-MS and a comparison of extract Cleanup by Immunoaffinity Chromatography (IAC) and Solid Phase Extraction (SPE)

Popis výsledku v původním jazyce

Isoflavonoids are plant phenolics known for their multiple pharmacological activities such as estrogenic, antibacterial, antioxidative etc. While legumes are their main source, the presence of isoflavonoids has been reported in about fifty other families. In non-legumes, isoflavonoids may be present in low concentrations, which complicates their detection and identification in complex biological matrixes. Two methods of sample clean-up prior to HPLC-ESI-MS, i.e. SPE (Solid Phase Extraction) and IAC (Immunoaffinity Chromatography), were compared. Conventional C18 reversed-phase sorbent cartridges (Waters Oasis, 30 ?m) were used for SPE. Immunosorbents for IAC were prepared by immobilisation of polyclonal rabbit antibodies against isoflavonoid-BSA (i.e.genistein, biochanin A and daidzein) conjugates on Affi-Gel 10 solid support (Bio-Rad Laboratories). A set of isoflavonoid standards and edible fig?s (Ficus carica) leaves and fruit ethanolic extracts were cleaned-up by two above mentione

Název v anglickém jazyce

Analysis of isoflavonoids in Ficus carica by HPLC-MS and a comparison of extract Cleanup by Immunoaffinity Chromatography (IAC) and Solid Phase Extraction (SPE)

Popis výsledku anglicky

Isoflavonoids are plant phenolics known for their multiple pharmacological activities such as estrogenic, antibacterial, antioxidative etc. While legumes are their main source, the presence of isoflavonoids has been reported in about fifty other families. In non-legumes, isoflavonoids may be present in low concentrations, which complicates their detection and identification in complex biological matrixes. Two methods of sample clean-up prior to HPLC-ESI-MS, i.e. SPE (Solid Phase Extraction) and IAC (Immunoaffinity Chromatography), were compared. Conventional C18 reversed-phase sorbent cartridges (Waters Oasis, 30 ?m) were used for SPE. Immunosorbents for IAC were prepared by immobilisation of polyclonal rabbit antibodies against isoflavonoid-BSA (i.e.genistein, biochanin A and daidzein) conjugates on Affi-Gel 10 solid support (Bio-Rad Laboratories). A set of isoflavonoid standards and edible fig?s (Ficus carica) leaves and fruit ethanolic extracts were cleaned-up by two above mentione

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F09%2F0994" target="_blank" >GA525/09/0994: Chemotaxonomie isoflavonoidů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Polyphenols Communications 2010

ISBN

978-2-7380-1282-1

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

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Název nakladatele

Groupe Polyphénols

Místo vydání

Montpellier

Místo konání akce

Montpellier, Francie

Datum konání akce

1. 1. 2010

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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