The multivariate analysis of vibrational spectra of wood mushrooms of genera Phellinus and Inonotus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F09%3A00022129" target="_blank" >RIV/60461373:22330/09:00022129 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The multivariate analysis of vibrational spectra of wood mushrooms of genera Phellinus and Inonotus

Popis výsledku v původním jazyce

A set of samples (fruit bodies and submersion cultures) originated from wood mushrooms of genera Phellinus and Inonotus were analysed by FT-IR (transmittance of KBr pellets, ATR) and FT-NIR (diffuse reflectance) spectroscopy. Purification of some samplesby washing with dilute HCl in 80% ethanol led to some spectroscopic changes, which were more pronounced for submersion cultures. Normalised spectra were used for hierarchy cluster analysis (HCA), and second derivatives (SD) of these spectra for principal component analysis (PCA). Fruit bodies and submersion cultures were successfully discriminated by HCA (Ward method, Manhattan distances) and by a principal component (PC) 1, which accounted for 46 % (FT-IR transmittance), 61 % (FT-IR ATR) and 78 % (FT-NIR) of the variation in all variables. The major PC1 extremes contributing to discrimination were assigned to proteins, aromatics and polysaccharides. Raw and purified submersion cultures of genus Phellinus were discriminated by PC2. The

Název v anglickém jazyce

The multivariate analysis of vibrational spectra of wood mushrooms of genera Phellinus and Inonotus

Popis výsledku anglicky

A set of samples (fruit bodies and submersion cultures) originated from wood mushrooms of genera Phellinus and Inonotus were analysed by FT-IR (transmittance of KBr pellets, ATR) and FT-NIR (diffuse reflectance) spectroscopy. Purification of some samplesby washing with dilute HCl in 80% ethanol led to some spectroscopic changes, which were more pronounced for submersion cultures. Normalised spectra were used for hierarchy cluster analysis (HCA), and second derivatives (SD) of these spectra for principal component analysis (PCA). Fruit bodies and submersion cultures were successfully discriminated by HCA (Ward method, Manhattan distances) and by a principal component (PC) 1, which accounted for 46 % (FT-IR transmittance), 61 % (FT-IR ATR) and 78 % (FT-NIR) of the variation in all variables. The major PC1 extremes contributing to discrimination were assigned to proteins, aromatics and polysaccharides. Raw and purified submersion cultures of genus Phellinus were discriminated by PC2. The

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GC521%2F07%2FJ039" target="_blank" >GC521/07/J039: Dřevní houby z rodů Phellinus a Inonotus: taxonomie, ekologie, molekulární fylogeneze, produkce polysacharidů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 5th International Conference on Polysaccharides-Glycoscience

ISBN

978-80-86238-72-2

ISSN

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e-ISSN

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Počet stran výsledku

15

Strana od-do

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Název nakladatele

Česká společnost chemická

Místo vydání

Praha

Místo konání akce

Praha, Česká republika

Datum konání akce

11. 11. 2009

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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