Acrylamide Analysis in Various Matrices Employing HPLC-MS/MS and UHPLC-TOF MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F10%3A00024392" target="_blank" >RIV/60461373:22330/10:00024392 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Acrylamide Analysis in Various Matrices Employing HPLC-MS/MS and UHPLC-TOF MS

Popis výsledku v původním jazyce

Occurrence of acrylamide in heat processed foodstuffs represents an issue of health concern. To monitor levels of this processing contaminant in various matrices (potato or cereal based products, coffee etc.), reliable analytical methods are needed. Currently, the most routinely used technique for acrylamide determination is high performance liquid chromatography coupled to tandem in space mass spectrometry (HPLC-MS/MS). Typically, relatively laborious sample preparation procedure employing two-step solid phase extraction is used prior to instrumental analysis. Our significantly simplified sample preparation procedure based on QuEChERS approach employs (i) addition of acetonitrile to primary aqueous extract and (ii) induction of phase separation by addition of salts. Isotope dilution technique (13C3-acrylamide as an internal standard) is employed to compensate both potential losses of analyte and matrix-induced chromatographic response enhancement. The QuEChERS approach was compared to

Název v anglickém jazyce

Acrylamide Analysis in Various Matrices Employing HPLC-MS/MS and UHPLC-TOF MS

Popis výsledku anglicky

Occurrence of acrylamide in heat processed foodstuffs represents an issue of health concern. To monitor levels of this processing contaminant in various matrices (potato or cereal based products, coffee etc.), reliable analytical methods are needed. Currently, the most routinely used technique for acrylamide determination is high performance liquid chromatography coupled to tandem in space mass spectrometry (HPLC-MS/MS). Typically, relatively laborious sample preparation procedure employing two-step solid phase extraction is used prior to instrumental analysis. Our significantly simplified sample preparation procedure based on QuEChERS approach employs (i) addition of acetonitrile to primary aqueous extract and (ii) induction of phase separation by addition of salts. Isotope dilution technique (13C3-acrylamide as an internal standard) is employed to compensate both potential losses of analyte and matrix-induced chromatographic response enhancement. The QuEChERS approach was compared to

Druh

D - Stať ve sborníku

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/2B06168" target="_blank" >2B06168: Strategie zajištění chemické bezpečnosti tepelně zpracovaných výrobků z brambor a cereálií</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

6th International Student Conference Modern Analytical chemistry

ISBN

978-80-7444-005-2

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

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Název nakladatele

Univerzita Karlova v Praze

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

1. 1. 2010

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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