Larvae of fleshfly Neobellieria bullata as a source for novel antimicrobial peptides

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Larvae of fleshfly Neobellieria bullata as a source for novel antimicrobial peptides

Popis výsledku v původním jazyce

The resistant strains of microorganisms are increasing threat for public health. Antimicrobial peptides from natural sources display a wide spectrum of activity. In our lab several peptide fraction with antimicrobial acivity against grampositive and gramnegative bacteria and fungi from larvae of fleshfly Neobellieria bullata were isolated. Presence of peptides was proved by tricine electrophoresis, molecular weights and sequences were determined by MS and N-terminal Edman sequencing. In comparison withdatabase sequences we obtained structures present in mainly insect and amphibian antimicrobial peptides, but total matches were not corresponding more than in 36%. Upon the obtained sequence motifs we designed structures of several synthetic peptides. These were synthetised on the Rink amide MBHA resin. Synthesis was performed according Fmoc/PyBOP-DIEA protocol. Activity of these peptides was determined.

Název v anglickém jazyce

Larvae of fleshfly Neobellieria bullata as a source for novel antimicrobial peptides

Popis výsledku anglicky

The resistant strains of microorganisms are increasing threat for public health. Antimicrobial peptides from natural sources display a wide spectrum of activity. In our lab several peptide fraction with antimicrobial acivity against grampositive and gramnegative bacteria and fungi from larvae of fleshfly Neobellieria bullata were isolated. Presence of peptides was proved by tricine electrophoresis, molecular weights and sequences were determined by MS and N-terminal Edman sequencing. In comparison withdatabase sequences we obtained structures present in mainly insect and amphibian antimicrobial peptides, but total matches were not corresponding more than in 36%. Upon the obtained sequence motifs we designed structures of several synthetic peptides. These were synthetised on the Rink amide MBHA resin. Synthesis was performed according Fmoc/PyBOP-DIEA protocol. Activity of these peptides was determined.

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Collection Symposium Series: Biologically Active Peptides

ISBN

978-80-86241-44-9

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

100-102

Název nakladatele

Ústav organické chemie a biochemie AV ČR

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

27. 4. 2011

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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