Antibacterial effect of compounds of peptide nature contained in aqueous extract of Brassica napus, Solanum lycopersicum and Tetragonia tetragonioides leaves

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00506427" target="_blank" >RIV/61388963:_____/15:00506427 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.jmbfs.org/issue/april-may-2015-vol-4-no/jmbfs-0684-neubauerova/?issue_id=3869&article_id=10" target="_blank" >https://www.jmbfs.org/issue/april-may-2015-vol-4-no/jmbfs-0684-neubauerova/?issue_id=3869&article_id=10</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15414/jmbfs.2015.4.5.427-433" target="_blank" >10.15414/jmbfs.2015.4.5.427-433</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Antibacterial effect of compounds of peptide nature contained in aqueous extract of Brassica napus, Solanum lycopersicum and Tetragonia tetragonioides leaves

Popis výsledku v původním jazyce

Treatment of infections caused by pathogenic bacteria is still harder. Due to increasing number of microbial species resistant against so far invented antibiotics. This presents great problem for public health. One of the potential solutions seems to be antimicrobial peptides. Those peptides are synthetized in all organisms as a part of innate immunity with rapid mode of antimicrobial action. Lot of them have been isolated from bacteria, plants, insects and mammals as well. Our project was aimed on finding such peptides in plant extracts, respectively in leaves of Brassica napus (canola), Solanum lycopersicum (tomato) and Tetragonia tetragonioides (New Zealand spinach). We used several separation techniques to obtain fractions containing compounds of peptide nature with hydrophobic character. Antimicrobial activity of these fractions was tested against several gram-positive and gram-negative bacteria. Mass spectrometry analysis of antimicrobial active fractions proved presence of low molecular peptides with molecular masses 1.9 - 4.9 kDa and a partial amino acid sequence in hydrophobic part of Tetragonia extract. In hydrophilic fraction of the Solanum extract with proved antibacterial activity two patogenesis-related proteins with antifungal activity NP24 and TPM-1 were detected.

Název v anglickém jazyce

Antibacterial effect of compounds of peptide nature contained in aqueous extract of Brassica napus, Solanum lycopersicum and Tetragonia tetragonioides leaves

Popis výsledku anglicky

Treatment of infections caused by pathogenic bacteria is still harder. Due to increasing number of microbial species resistant against so far invented antibiotics. This presents great problem for public health. One of the potential solutions seems to be antimicrobial peptides. Those peptides are synthetized in all organisms as a part of innate immunity with rapid mode of antimicrobial action. Lot of them have been isolated from bacteria, plants, insects and mammals as well. Our project was aimed on finding such peptides in plant extracts, respectively in leaves of Brassica napus (canola), Solanum lycopersicum (tomato) and Tetragonia tetragonioides (New Zealand spinach). We used several separation techniques to obtain fractions containing compounds of peptide nature with hydrophobic character. Antimicrobial activity of these fractions was tested against several gram-positive and gram-negative bacteria. Mass spectrometry analysis of antimicrobial active fractions proved presence of low molecular peptides with molecular masses 1.9 - 4.9 kDa and a partial amino acid sequence in hydrophobic part of Tetragonia extract. In hydrophilic fraction of the Solanum extract with proved antibacterial activity two patogenesis-related proteins with antifungal activity NP24 and TPM-1 were detected.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiology, Biotechnology and Food Sciences

ISSN

1338-5178

e-ISSN

—

Svazek periodika

4

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

7

Strana od-do

427-433

Kód UT WoS článku

000458093400011

EID výsledku v databázi Scopus

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