Simultaneous screening for T-2/HT-2 and deoxynivalenol in cereals using a surface plasmon resonance immunoassay

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F12%3A43894970" target="_blank" >RIV/60461373:22330/12:43894970 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.3920/WMJ2011.1351" target="_blank" >http://dx.doi.org/10.3920/WMJ2011.1351</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3920/WMJ2011.1351" target="_blank" >10.3920/WMJ2011.1351</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Simultaneous screening for T-2/HT-2 and deoxynivalenol in cereals using a surface plasmon resonance immunoassay

Popis výsledku v původním jazyce

This manuscript describes a rapid surface plasmon resonance (SPR) immunoassay for the simultaneous determination of the sum of T-2/HT-2 toxins (T-2/HT-2) and deoxynivalenol (DON), in cereals and cereal-based products. The assay is based on an inhibitionformat employing a monoclonal antibody raised against HT-2 with cross reactivity to T-2 and a polyclonal antibody raised against DON, thereby enabling the detection of the three trichothecene mycotoxins (types A and B). The surface chemistry involved anequal mixture of HT-2 and DON covalently coupled onto a high capacity COONS sensor chip. Using the specified antibodies and a mixed toxin sensor surface, and running calibration curves (HT-2 and DON) and samples in parallel it has been proven that it isfeasible to develop a multiplex assay on this SPR platform. In-house validation has shown limits of detection of 12, 1 and 29 mu g/kg for DON and 31, 47 and 36 mu g/kg for HT-2 in wheat, breakfast cereal and maize-based baby food, respect

Název v anglickém jazyce

Simultaneous screening for T-2/HT-2 and deoxynivalenol in cereals using a surface plasmon resonance immunoassay

Popis výsledku anglicky

This manuscript describes a rapid surface plasmon resonance (SPR) immunoassay for the simultaneous determination of the sum of T-2/HT-2 toxins (T-2/HT-2) and deoxynivalenol (DON), in cereals and cereal-based products. The assay is based on an inhibitionformat employing a monoclonal antibody raised against HT-2 with cross reactivity to T-2 and a polyclonal antibody raised against DON, thereby enabling the detection of the three trichothecene mycotoxins (types A and B). The surface chemistry involved anequal mixture of HT-2 and DON covalently coupled onto a high capacity COONS sensor chip. Using the specified antibodies and a mixed toxin sensor surface, and running calibration curves (HT-2 and DON) and samples in parallel it has been proven that it isfeasible to develop a multiplex assay on this SPR platform. In-house validation has shown limits of detection of 12, 1 and 29 mu g/kg for DON and 31, 47 and 36 mu g/kg for HT-2 in wheat, breakfast cereal and maize-based baby food, respect

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

WORLD MYCOTOXIN JOURNAL

ISSN

1875-0710

e-ISSN

—

Svazek periodika

5

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

117-126

Kód UT WoS článku

000304585700002

EID výsledku v databázi Scopus

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