Peptidoglycan Hydrolases as Species-Specific Markers to Differentiate Lactobacillus helveticus from Lactobacillus gallinarum and Other Closely Related Homofermentative Lactobacilli

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43895925" target="_blank" >RIV/60461373:22330/14:43895925 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007/s00284-013-0512-5" target="_blank" >http://link.springer.com/article/10.1007/s00284-013-0512-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00284-013-0512-5" target="_blank" >10.1007/s00284-013-0512-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Peptidoglycan Hydrolases as Species-Specific Markers to Differentiate Lactobacillus helveticus from Lactobacillus gallinarum and Other Closely Related Homofermentative Lactobacilli

Popis výsledku v původním jazyce

We propose a new method that allows accurate discrimination of Lactobacillus helveticus from other closely related homofermentative lactobacilli, especially Lactobacillus gallinarum. This method is based on the amplification by PCR of two peptidoglycan hydrolytic genes, Lhv_0190 and Lhv_0191. These genes are ubiquitous and show high homology at the intra-species level. The PCR method gave two specific PCR products, of 542 bp and 747 bp, for 25 L. helveticus strains coming from various sources. For L. gallinarum, two amplicons were obtained, the specific 542 bp amplicon and another one with a size greater than 1500 bp. No specific PCR products were obtained for 12 other closely related species of lactobacilli, including the Lactobacillus acidophilus complex, Lactobacillus delbrueckii, and Lactobacillus ultunensis. The developed PCR method provided rapid, precise and easy identification of L. helveticus. Moreover, it enabled differentiation between the two closely phylogenetically relate

Název v anglickém jazyce

Peptidoglycan Hydrolases as Species-Specific Markers to Differentiate Lactobacillus helveticus from Lactobacillus gallinarum and Other Closely Related Homofermentative Lactobacilli

Popis výsledku anglicky

We propose a new method that allows accurate discrimination of Lactobacillus helveticus from other closely related homofermentative lactobacilli, especially Lactobacillus gallinarum. This method is based on the amplification by PCR of two peptidoglycan hydrolytic genes, Lhv_0190 and Lhv_0191. These genes are ubiquitous and show high homology at the intra-species level. The PCR method gave two specific PCR products, of 542 bp and 747 bp, for 25 L. helveticus strains coming from various sources. For L. gallinarum, two amplicons were obtained, the specific 542 bp amplicon and another one with a size greater than 1500 bp. No specific PCR products were obtained for 12 other closely related species of lactobacilli, including the Lactobacillus acidophilus complex, Lactobacillus delbrueckii, and Lactobacillus ultunensis. The developed PCR method provided rapid, precise and easy identification of L. helveticus. Moreover, it enabled differentiation between the two closely phylogenetically relate

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/QI101B090" target="_blank" >QI101B090: Nové postupy produkce funkčních cereálních a mléčných potravin a funkčních nápojů s obsahem bioaktivních složek z vybraných rostlinných a živočišných zemědělských surovin s využitím probiotických mikroorganismů a postupy posuzování jejich kvality</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

CURRENT MICROBIOLOGY

ISSN

0343-8651

e-ISSN

—

Svazek periodika

68

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

551-557

Kód UT WoS článku

000331006200019

EID výsledku v databázi Scopus

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