Characterization of transgenic tobacco plants containing bacterial bphC gene and study of their phytoremediation ability

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43897358" target="_blank" >RIV/60461373:22330/14:43897358 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1080/15226514.2013.810575" target="_blank" >http://dx.doi.org/10.1080/15226514.2013.810575</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/15226514.2013.810575" target="_blank" >10.1080/15226514.2013.810575</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of transgenic tobacco plants containing bacterial bphC gene and study of their phytoremediation ability

Popis výsledku v původním jazyce

Genetically modified plants can serve as an efficient tool for remediation of diverse dangerous pollutants of the environment such as pesticides, heavy metals, explosives and persistent organic compounds. Transgenic lines of Nicotiana tabacum containingbacterial bphC gene from the degradation pathway of polychlorinated biphenyls (PCBs) were tested. The product of the bphC gene-enzyme 2,3-dihydroxybiphenyl-1,2-dioxygenase is responsible for cleaving of the biphenyl ring. The presence of bphC gene in transgenic plants was detected on DNA, RNA and protein level. The expression of the bphC/His gene was verified after purification of the enzyme from plants by affinity chromatography followed by a Western blot and immunochemical assay. The enzyme activity of isolated protein was detected. Efficient transformation of 2,3-DHB by transgenic plants was achieved and the lines also exhibited high production of biomass. The transgenic plants were more tolerant to the commercial PCBs mixture Delor

Název v anglickém jazyce

Characterization of transgenic tobacco plants containing bacterial bphC gene and study of their phytoremediation ability

Popis výsledku anglicky

Genetically modified plants can serve as an efficient tool for remediation of diverse dangerous pollutants of the environment such as pesticides, heavy metals, explosives and persistent organic compounds. Transgenic lines of Nicotiana tabacum containingbacterial bphC gene from the degradation pathway of polychlorinated biphenyls (PCBs) were tested. The product of the bphC gene-enzyme 2,3-dihydroxybiphenyl-1,2-dioxygenase is responsible for cleaving of the biphenyl ring. The presence of bphC gene in transgenic plants was detected on DNA, RNA and protein level. The expression of the bphC/His gene was verified after purification of the enzyme from plants by affinity chromatography followed by a Western blot and immunochemical assay. The enzyme activity of isolated protein was detected. Efficient transformation of 2,3-DHB by transgenic plants was achieved and the lines also exhibited high production of biomass. The transgenic plants were more tolerant to the commercial PCBs mixture Delor

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/LH12087" target="_blank" >LH12087: Mikrobiální diversita a interakce rostlina-mikroorganismus u rostlin remediujících kontaminanty ? optimalizace symbiotického synergismu u fytoremediací</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Phytoremediation

ISSN

1522-6514

e-ISSN

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Svazek periodika

16

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

937-946

Kód UT WoS článku

000330020500006

EID výsledku v databázi Scopus

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