Effects of ampicilin and vancomycin on Staphylococcus aureus biofilms

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43898396" target="_blank" >RIV/60461373:22330/14:43898396 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.agriculturejournals.cz/publicFiles/119577.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/119577.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effects of ampicilin and vancomycin on Staphylococcus aureus biofilms

Popis výsledku v původním jazyce

The collection of 23 coagulase positive Staphylococcus aureus strains isolated mainly from food in the Czech Republic were tested on the ability to form biofilms in the presence of ampicilin and vancomycin. The antimicrobial sensitivity (16 antibiotics)was determined in all strains by the standard disc diffusion method on Mueller-Hinton agar plates (NCCLS). The resistence to ampicilin was found in 16 strains (69.5%), all strains were susceptible to vancomycin. The formation of biofilm was conducted in96-well, polystyrene microtiter plates COSTAR 3797 in tryptic soy broth (TSB) with 1% glucose for 2424 h at 30oC. Staining with crystal violet (0.1%) was used for biofilm quantification. Ampicillin( 0.5, 2,and 4 mg/l) and vancomycin ( 32, 64 and 128 mg/l) were added: (i) direct addition of the agent to the well at zero time, (ii) after 24 h to washed well, (iii) after 24 h directly to the well with cell suspension. The tested types of ampicilin treatment did not confirm the impact to res

Název v anglickém jazyce

Effects of ampicilin and vancomycin on Staphylococcus aureus biofilms

Popis výsledku anglicky

The collection of 23 coagulase positive Staphylococcus aureus strains isolated mainly from food in the Czech Republic were tested on the ability to form biofilms in the presence of ampicilin and vancomycin. The antimicrobial sensitivity (16 antibiotics)was determined in all strains by the standard disc diffusion method on Mueller-Hinton agar plates (NCCLS). The resistence to ampicilin was found in 16 strains (69.5%), all strains were susceptible to vancomycin. The formation of biofilm was conducted in96-well, polystyrene microtiter plates COSTAR 3797 in tryptic soy broth (TSB) with 1% glucose for 2424 h at 30oC. Staining with crystal violet (0.1%) was used for biofilm quantification. Ampicillin( 0.5, 2,and 4 mg/l) and vancomycin ( 32, 64 and 128 mg/l) were added: (i) direct addition of the agent to the well at zero time, (ii) after 24 h to washed well, (iii) after 24 h directly to the well with cell suspension. The tested types of ampicilin treatment did not confirm the impact to res

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Food Sciences

ISSN

1212-1800

e-ISSN

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Svazek periodika

32

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

137-144

Kód UT WoS článku

000335282200004

EID výsledku v databázi Scopus

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