Detection of some phenotypic and genotypic characteristics of Staphylococcus aureus isolated from food items in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43898413" target="_blank" >RIV/60461373:22330/14:43898413 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/14:#0001186

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs13213-014-0802-6" target="_blank" >http://link.springer.com/article/10.1007%2Fs13213-014-0802-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13213-014-0802-6" target="_blank" >10.1007/s13213-014-0802-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of some phenotypic and genotypic characteristics of Staphylococcus aureus isolated from food items in the Czech Republic

Popis výsledku v původním jazyce

Staphylococcus aureus is an important foodborne pathogen and can produce a wide range enterotoxins, which contribute to food poisoning. The aim of this study was to interrogate foodborne strains of S. aureus for phenotypic and genotypic characteristics.Strains were screened for enterotoxins, hemolysins and antimicrobial resistance, and the genetic relationship between strains was described after pulsed-field gel electrophoresis (PFGE) analysis. Of the S. aureus strains, 82.8%(n=93) harboured one or more of the following enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, sej); 39.8 % of strains demonstrated se genes and 43 % carried from two to five se genes, while 17.2 % of the strains possessed none of the genes examined. The most commonly detected toxin genes were sea, seb, sec and seg. The presence of genes coding for antibiotic resistance such as blaZ, vanA, vanB and mecA was investigated by polymerase chain reaction (PCR). Seventy-two strains carrying the blaZ gene exhibi

Název v anglickém jazyce

Detection of some phenotypic and genotypic characteristics of Staphylococcus aureus isolated from food items in the Czech Republic

Popis výsledku anglicky

Staphylococcus aureus is an important foodborne pathogen and can produce a wide range enterotoxins, which contribute to food poisoning. The aim of this study was to interrogate foodborne strains of S. aureus for phenotypic and genotypic characteristics.Strains were screened for enterotoxins, hemolysins and antimicrobial resistance, and the genetic relationship between strains was described after pulsed-field gel electrophoresis (PFGE) analysis. Of the S. aureus strains, 82.8%(n=93) harboured one or more of the following enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, sej); 39.8 % of strains demonstrated se genes and 43 % carried from two to five se genes, while 17.2 % of the strains possessed none of the genes examined. The most commonly detected toxin genes were sea, seb, sec and seg. The presence of genes coding for antibiotic resistance such as blaZ, vanA, vanB and mecA was investigated by polymerase chain reaction (PCR). Seventy-two strains carrying the blaZ gene exhibi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Annals of Microbiology

ISSN

1590-4261

e-ISSN

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Svazek periodika

64

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

12

Strana od-do

1587-1596

Kód UT WoS článku

000345292900013

EID výsledku v databázi Scopus

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