Molecular analysis of Staphylococcus aureus pathogenicity islands (SaPI) and their superantigens combination of food samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43898424" target="_blank" >RIV/60461373:22330/14:43898424 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0167701214003042" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0167701214003042</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mimet.2014.10.014" target="_blank" >10.1016/j.mimet.2014.10.014</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular analysis of Staphylococcus aureus pathogenicity islands (SaPI) and their superantigens combination of food samples

Popis výsledku v původním jazyce

Staphylococcus aureus produces a wide variety of superantigenic activity Staphylococcal enterotoxins (SE) and they are a major cause of food poisoning. These superantigens are associated with mobile genetic elements such as plasmids, prophages and S. aureus pathogenicity islands (SaPI). The presence of well-known eight SaPI integrase and 13 enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sel, sek, seq, and tst) in 93 S. aureus strains were investigated. All S. aureus isolates were characterized by pulsed-field gel electrophoresis (PFGE), and the genes were detected using five sets of multiplex PCR (mPCR). The most predominant toxin genes were sea (19%), seb (15%), sec (54%), sell (48%), selk (46%), selq (52%), seg (22%), and sei (19%).Analysis showed that many S. aureus isolates harbored multiple toxin genes. An mPCR-based assay was developed for the determination of all SaPI and their superantigen gene combinations. Twenty three isolates revealed the gene combination

Název v anglickém jazyce

Molecular analysis of Staphylococcus aureus pathogenicity islands (SaPI) and their superantigens combination of food samples

Popis výsledku anglicky

Staphylococcus aureus produces a wide variety of superantigenic activity Staphylococcal enterotoxins (SE) and they are a major cause of food poisoning. These superantigens are associated with mobile genetic elements such as plasmids, prophages and S. aureus pathogenicity islands (SaPI). The presence of well-known eight SaPI integrase and 13 enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sel, sek, seq, and tst) in 93 S. aureus strains were investigated. All S. aureus isolates were characterized by pulsed-field gel electrophoresis (PFGE), and the genes were detected using five sets of multiplex PCR (mPCR). The most predominant toxin genes were sea (19%), seb (15%), sec (54%), sell (48%), selk (46%), selq (52%), seg (22%), and sei (19%).Analysis showed that many S. aureus isolates harbored multiple toxin genes. An mPCR-based assay was developed for the determination of all SaPI and their superantigen gene combinations. Twenty three isolates revealed the gene combination

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/QJ1210300" target="_blank" >QJ1210300: Systémy jištění kvality a bezpečnosti mlékárenských výrobků vhodnými metodami aplikovatelnými v praxi</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiological Methods

ISSN

0167-7012

e-ISSN

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Svazek periodika

107

Číslo periodika v rámci svazku

DEC 2014

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

197-204

Kód UT WoS článku

000347605900033

EID výsledku v databázi Scopus

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