Detection of meat adulteration: Use of efficient and routine-suited multiplex polymerase chain reaction-based methods for species authentication and quantification in meat products

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F18%3A43917324" target="_blank" >RIV/60461373:22330/18:43917324 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22810/18:43917324

Výsledek na webu

<a href="http://www.vup.sk/index.php?mainID=2&navID=36&version=2&volume=0&article=2116&start" target="_blank" >http://www.vup.sk/index.php?mainID=2&navID=36&version=2&volume=0&article=2116&start</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of meat adulteration: Use of efficient and routine-suited multiplex polymerase chain reaction-based methods for species authentication and quantification in meat products

Popis výsledku v původním jazyce

The increase in the extent of meat adulteration is the reason for a need for an effective method for authentication of meat products. DNA-based polymerase chain reaction (PCR) is a well suited alternative for this purpose. Furthermore, the method facilitates quantification of animal DNA in meat products based on the correlation between target copy amounts and cycle numbers in quantitative PCR. We designed and experimentally verified PCR primer systems for identification of beef, pork, horse and poultry (chicken, turkey) meat. Mitochondrial and chromosomal markers were used. The mitochondrial cytochrome b gene was used as a marker for qualitative multiplex endpoint PCR and single-copy chromosomal genes (cyclic-GMP-phosphodiesterase gene for cattle, beta-actin gene for pig, interleukin-2 gene for chicken, myostatin gene for mammals and poultry) were used for multiplex quantitative PCR analyses. The reliability of both methods was confirmed by analysing of mixed samples prepared with or without heat treatment. The methods were then applied to 14 commercially available products typical for the Czech Republic, including sausages or salami. Discrepancies were observed between the DNA analysis and the meat content declared for the tested products, as two of the samples did not correspond to qualitative requirements and other four failed to meet quantitative requirements. The proposed PCR-based methodology was shown to be useful for the disclosure of meat adulteration.

Název v anglickém jazyce

Detection of meat adulteration: Use of efficient and routine-suited multiplex polymerase chain reaction-based methods for species authentication and quantification in meat products

Popis výsledku anglicky

The increase in the extent of meat adulteration is the reason for a need for an effective method for authentication of meat products. DNA-based polymerase chain reaction (PCR) is a well suited alternative for this purpose. Furthermore, the method facilitates quantification of animal DNA in meat products based on the correlation between target copy amounts and cycle numbers in quantitative PCR. We designed and experimentally verified PCR primer systems for identification of beef, pork, horse and poultry (chicken, turkey) meat. Mitochondrial and chromosomal markers were used. The mitochondrial cytochrome b gene was used as a marker for qualitative multiplex endpoint PCR and single-copy chromosomal genes (cyclic-GMP-phosphodiesterase gene for cattle, beta-actin gene for pig, interleukin-2 gene for chicken, myostatin gene for mammals and poultry) were used for multiplex quantitative PCR analyses. The reliability of both methods was confirmed by analysing of mixed samples prepared with or without heat treatment. The methods were then applied to 14 commercially available products typical for the Czech Republic, including sausages or salami. Discrepancies were observed between the DNA analysis and the meat content declared for the tested products, as two of the samples did not correspond to qualitative requirements and other four failed to meet quantitative requirements. The proposed PCR-based methodology was shown to be useful for the disclosure of meat adulteration.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/QJ1530272" target="_blank" >QJ1530272: Komplexní strategie pro efektivní odhalování falšování potravin v řetězci (prvo)výroba - spotřebitel</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food and Nutrition Research

ISSN

1336-8672

e-ISSN

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Svazek periodika

57

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

12

Strana od-do

351-362

Kód UT WoS článku

000451926900004

EID výsledku v databázi Scopus

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