USE OF DNA ANALYSIS FOR THE STUDY OF MEAT AND FISH FRAUD

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919822" target="_blank" >RIV/60461373:22330/19:43919822 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

USE OF DNA ANALYSIS FOR THE STUDY OF MEAT AND FISH FRAUD

Popis výsledku v původním jazyce

Food fraud is the current and serious problem on a global scale. Fish, meat and their products are one of the most expensive foods and therefore fall into the category of the adulterated commodities. One common way of deceiving customers is to replace high-quality meat with a less valuable and/or incorrect labeling of the product. Currently, many methods for determining species origin of meat exist. These include molecular-biological methods using DNA analysis, which allow very precise identification of the animal species used in food production.In this work, the polymerase chain reaction (PCR) was successfully used for identification of fish from the Scombridae family (mackerel, tuna, bonito), meat (cattle, pig, horse, chicken, duck, turkey) and products purchased in commercial sources in the Czech Republic. The multiplex PCR method was used for the qualitative determination of meat content in products. The meat of cattle, pigs, poultry, and horses was detected using primers complementary to the gene coding for cytochrome b (mitochondrial DNA). Primers specifically amplifying interleukin II (nuclear DNA) were used to differentiate poultry; species identification of fish was performed by analyzing the sequence of the parvalbumin gene (nuclear DNA). Quantitative multiplex PCR with real-time fluorescence detection (mqPCR) was used to quantify DNA of selected meat species (beef, pork, and chicken) in meat products. In the mqPCR reaction, primers were used in combination with appropriate hydrolysis probes complementary to the DNA encoding the porcine beta-actin, beef cyclic-GMP-phosphodiesterase genes, chicken interleukin II and the gene encoding myostatin, which is a universal marker for mammals and poultry. In some cases, discrepancies between the results of DNA analysis and content of the sample declared by producer were determined.

Název v anglickém jazyce

USE OF DNA ANALYSIS FOR THE STUDY OF MEAT AND FISH FRAUD

Popis výsledku anglicky

Food fraud is the current and serious problem on a global scale. Fish, meat and their products are one of the most expensive foods and therefore fall into the category of the adulterated commodities. One common way of deceiving customers is to replace high-quality meat with a less valuable and/or incorrect labeling of the product. Currently, many methods for determining species origin of meat exist. These include molecular-biological methods using DNA analysis, which allow very precise identification of the animal species used in food production.In this work, the polymerase chain reaction (PCR) was successfully used for identification of fish from the Scombridae family (mackerel, tuna, bonito), meat (cattle, pig, horse, chicken, duck, turkey) and products purchased in commercial sources in the Czech Republic. The multiplex PCR method was used for the qualitative determination of meat content in products. The meat of cattle, pigs, poultry, and horses was detected using primers complementary to the gene coding for cytochrome b (mitochondrial DNA). Primers specifically amplifying interleukin II (nuclear DNA) were used to differentiate poultry; species identification of fish was performed by analyzing the sequence of the parvalbumin gene (nuclear DNA). Quantitative multiplex PCR with real-time fluorescence detection (mqPCR) was used to quantify DNA of selected meat species (beef, pork, and chicken) in meat products. In the mqPCR reaction, primers were used in combination with appropriate hydrolysis probes complementary to the DNA encoding the porcine beta-actin, beef cyclic-GMP-phosphodiesterase genes, chicken interleukin II and the gene encoding myostatin, which is a universal marker for mammals and poultry. In some cases, discrepancies between the results of DNA analysis and content of the sample declared by producer were determined.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40101 - Agriculture

Projekt

<a href="/cs/project/QK1910231" target="_blank" >QK1910231: Nové přístupy k průkazu falšování rybího masa pomocí genomové DNA</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů