Immunochemical techniques for anabolic androgenic steroid – matrix effects study for food supplements

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919744" target="_blank" >RIV/60461373:22330/19:43919744 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22810/19:43919744

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs00217-018-3204-3" target="_blank" >https://link.springer.com/article/10.1007%2Fs00217-018-3204-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00217-018-3204-3" target="_blank" >10.1007/s00217-018-3204-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immunochemical techniques for anabolic androgenic steroid – matrix effects study for food supplements

Popis výsledku v původním jazyce

Anabolic androgenic steroid contaminations in food supplements are common, and their consumption may lead to positive doping results. One of such anabolic steroid is mesterolone selected as an analyte for the present study. Some contaminated supplements cannot be detected by traditional methods due to the matrix effect. Two variants of indirect competitive immunochemical methodan enzyme-linked immunosorbent assay and an immunochromatographic method were developed using rabbit polyclonal antibodies and corresponding mesterolone conjugated at position C-17 to bovine serum albumin. Under optimal experimental conditions, the ELISA achieved the limit of the detection value of 0.010 +/- 0.003ngmL(-1) and for LFIA visual detection limit was determined as concentration 50ngmL(-1). Both methods showed the mesterolone specificity of anti-mesterolone antibodies. The developed ELISA and LFIA were then applied to artificially contaminated food supplements. Before immunoassays, the samples were treated using a simple extraction protocol that did not include preliminary clean-up steps. For ELISA, a negative effect on the sensitivity of the method was observed for complex matrix samples. All type of analysed sample matrixes have minimal effect on LFIA sensitivity and, therefore, it could be successfully applied for the simple screening quality of food supplements.

Název v anglickém jazyce

Immunochemical techniques for anabolic androgenic steroid – matrix effects study for food supplements

Popis výsledku anglicky

Anabolic androgenic steroid contaminations in food supplements are common, and their consumption may lead to positive doping results. One of such anabolic steroid is mesterolone selected as an analyte for the present study. Some contaminated supplements cannot be detected by traditional methods due to the matrix effect. Two variants of indirect competitive immunochemical methodan enzyme-linked immunosorbent assay and an immunochromatographic method were developed using rabbit polyclonal antibodies and corresponding mesterolone conjugated at position C-17 to bovine serum albumin. Under optimal experimental conditions, the ELISA achieved the limit of the detection value of 0.010 +/- 0.003ngmL(-1) and for LFIA visual detection limit was determined as concentration 50ngmL(-1). Both methods showed the mesterolone specificity of anti-mesterolone antibodies. The developed ELISA and LFIA were then applied to artificially contaminated food supplements. Before immunoassays, the samples were treated using a simple extraction protocol that did not include preliminary clean-up steps. For ELISA, a negative effect on the sensitivity of the method was observed for complex matrix samples. All type of analysed sample matrixes have minimal effect on LFIA sensitivity and, therefore, it could be successfully applied for the simple screening quality of food supplements.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Food Research and Technology

ISSN

1438-2377

e-ISSN

—

Svazek periodika

245

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

1011-1019

Kód UT WoS článku

000467903900006

EID výsledku v databázi Scopus

2-s2.0-85057232767