Determination of carotenoids in flowers and food supplements by HPLC-DAD

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F20%3A43921061" target="_blank" >RIV/60461373:22330/20:43921061 - isvavai.cz</a>

Výsledek na webu

<a href="https://content.sciendo.com/configurable/contentpage/journals$002facs$002f13$002f1$002farticle-p6.xml" target="_blank" >https://content.sciendo.com/configurable/contentpage/journals$002facs$002f13$002f1$002farticle-p6.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/acs-2020-0002" target="_blank" >10.2478/acs-2020-0002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of carotenoids in flowers and food supplements by HPLC-DAD

Popis výsledku v původním jazyce

Marigold flowers (Tagetes patula and Calendula officinalis) were chosen for analysis because they are the most often used source of lutein and its isomer zeaxanthin for the production of food supplements on the Czech market. Direct extraction and extraction with alkaline hydrolysis were compared to detect free or bound carotenoids. For carotenoid separation, C18 and C30 columns were used. A new method for determination of cal oienoid content in food supplements in form of capsules has been developed and validated. All matrices were analysed by high-pet lot malice liquid chromatography with diode array detection (HPLC-DAD). It has been found that alkaline hydrolysis is required for both Marigold flowers and food supplements to release lutein from ester bonds to fatty acids. In Calendula officin,alislutein in the concentration of 807-1472 mg . kg(-1) of dry matter was detected. Tagetes patula has been identified as a better lutein source with the content of 5906-8677 mg . kg(-1) of dry matter. It has been found that the content of lutein and zeaxanthin in commercial food supplements (Lutein Complex Premium and Occutein Brillant) is consistent with the declared quantity. Linearity of the HPI.C-DAD method ranged from 0.1-20 mu g . mL(-1) with the limit of quantification (LOQ) of 1.7 mg . kg(-1) for lutein in Marigold flowers and 200 mg . kg(-1) in food supplements. Repeatability was 2.3 % for lutein in all tested matrices.

Název v anglickém jazyce

Determination of carotenoids in flowers and food supplements by HPLC-DAD

Popis výsledku anglicky

Marigold flowers (Tagetes patula and Calendula officinalis) were chosen for analysis because they are the most often used source of lutein and its isomer zeaxanthin for the production of food supplements on the Czech market. Direct extraction and extraction with alkaline hydrolysis were compared to detect free or bound carotenoids. For carotenoid separation, C18 and C30 columns were used. A new method for determination of cal oienoid content in food supplements in form of capsules has been developed and validated. All matrices were analysed by high-pet lot malice liquid chromatography with diode array detection (HPLC-DAD). It has been found that alkaline hydrolysis is required for both Marigold flowers and food supplements to release lutein from ester bonds to fatty acids. In Calendula officin,alislutein in the concentration of 807-1472 mg . kg(-1) of dry matter was detected. Tagetes patula has been identified as a better lutein source with the content of 5906-8677 mg . kg(-1) of dry matter. It has been found that the content of lutein and zeaxanthin in commercial food supplements (Lutein Complex Premium and Occutein Brillant) is consistent with the declared quantity. Linearity of the HPI.C-DAD method ranged from 0.1-20 mu g . mL(-1) with the limit of quantification (LOQ) of 1.7 mg . kg(-1) for lutein in Marigold flowers and 200 mg . kg(-1) in food supplements. Repeatability was 2.3 % for lutein in all tested matrices.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Chimica Slovaca

ISSN

1337-978X

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

7

Strana od-do

6-12

Kód UT WoS článku

000546283900002

EID výsledku v databázi Scopus

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