Lipidomic Analysis as a Tool for a Comprehensive Description of Atherosclerotic Plaques

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F20%3A43921090" target="_blank" >RIV/60461373:22330/20:43921090 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Lipidomic Analysis as a Tool for a Comprehensive Description of Atherosclerotic Plaques

Popis výsledku v původním jazyce

Atherosclerosis is a chronic inflammatory disease that causes lipids accumulation and the formation of fibrotic plaques in the walls of medium and large arteries. In this way, lipids are involved in the development of coronary heart disease and ischemic stroke, which belong among dominating causes of death in Western civilization. Considering the composition of the atherosclerotic plaque, lipidomic analysis was selected as a suitable tool for its study. To characterize lipid profile in an atherosclerotic plaque in a longitudinal direction, relevant strategy had to be implemented. In the first phase, the sample preparation of the atherosclerotic plaques (obtained in cooperation with Military University Hospital, Prague) was optimized. Then, a pilot experiment confirmed the different lipids composition in different parts of the plaque. The key experiment was a lipidomic analysis of the atherosclerotic plaque cuts, which revealed different distribution of lipids depending on the progression of stenosis and also between proximal and distal parts. Increased levels of oxidized free fatty acids and, conversely, a reduced content of plasmalogens were observed in the most affected parts of the plaque. In addition, a targeted screening of oxidized lipids was performed, and 46 oxidized lipids in total were detected in samples. MALDI-MS imaging was also used to describe the atherosclerotic plaque’s composition (in collaboration with Laboratory of Molecular Structure Characterization, Academy of Sciences of the Czech Republic). This challenging technique enabled to visualize the spatial distribution of compounds in a sample thus complemented information needed for atherosclerosis pathology study.

Název v anglickém jazyce

Lipidomic Analysis as a Tool for a Comprehensive Description of Atherosclerotic Plaques

Popis výsledku anglicky

Atherosclerosis is a chronic inflammatory disease that causes lipids accumulation and the formation of fibrotic plaques in the walls of medium and large arteries. In this way, lipids are involved in the development of coronary heart disease and ischemic stroke, which belong among dominating causes of death in Western civilization. Considering the composition of the atherosclerotic plaque, lipidomic analysis was selected as a suitable tool for its study. To characterize lipid profile in an atherosclerotic plaque in a longitudinal direction, relevant strategy had to be implemented. In the first phase, the sample preparation of the atherosclerotic plaques (obtained in cooperation with Military University Hospital, Prague) was optimized. Then, a pilot experiment confirmed the different lipids composition in different parts of the plaque. The key experiment was a lipidomic analysis of the atherosclerotic plaque cuts, which revealed different distribution of lipids depending on the progression of stenosis and also between proximal and distal parts. Increased levels of oxidized free fatty acids and, conversely, a reduced content of plasmalogens were observed in the most affected parts of the plaque. In addition, a targeted screening of oxidized lipids was performed, and 46 oxidized lipids in total were detected in samples. MALDI-MS imaging was also used to describe the atherosclerotic plaque’s composition (in collaboration with Laboratory of Molecular Structure Characterization, Academy of Sciences of the Czech Republic). This challenging technique enabled to visualize the spatial distribution of compounds in a sample thus complemented information needed for atherosclerosis pathology study.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30201 - Cardiac and Cardiovascular systems

Projekt

<a href="/cs/project/NV18-08-00149" target="_blank" >NV18-08-00149: Kritické zhodnocení lipidomu u pacientů s akutním koronárním syndromem a cévní mozkovou příhodou ve vztahu ke stupni oxidačního stresu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů