Critical assessment of Jamaican field cricket metabolome after rapeseed meal added to feed

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925442" target="_blank" >RIV/60461373:22330/22:43925442 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Critical assessment of Jamaican field cricket metabolome after rapeseed meal added to feed

Popis výsledku v původním jazyce

Constantly growing world population puts enormous pressure on food systems. One of the solutions how to supply the population with enough nutrients and especially proteins in the future is the production of edible insects. The main advantages of edible insects are high conversion of feed, small environmental footprint, and small demands on space and water. These advantages make edible insects a possible challenging sustainable alternative to traditional animal protein sources and make it a possible future common ingredient of food and feed. This potential usage of insects makes this commodity a growing concept and draws the attention of researchers who mainly focus on the impact of rearing conditions such as temperature on biomass gain, feed conversion, and nutritional properties. However, the effects of feed composition on the quality of edible insects are still quite unexplored, especially in terms of bioactive compounds and micronutrients profiles. Complementing knowledge in this field is particularly desirable for the future production of quality and safe edible insects.In this study, the effect of using by-product from rapeseed oil production (rapeseed meal) on metabolome of Jamaican field cricket (Gryllus assimilis) was investigated. Rapeseed meal was used in this experiment as a substitution for protein component (soya) in feed. Five groups of crickets differing in feed composition were analyzed. Attention was paid especially to the transfer of bioactive compounds. For the evaluation of the changes in cricket biomass, the metabolomic analysis using ultra-performance liquid chromatography coupled with high-resolution tandem mass spectrometry(U-HPLC-HRMS/MS) with subsequent use of chemometric tools (PCA, PLS-DA) was performed. Target screening of selected bioactive compounds such as sinapine or antinutritious glucosinolate goitrin was also performed.It was possible to distinguish the groups of Jamaican field cricket according to the feed composition and possible markers of rapeseed meal and soya in feed were identified. Transfer of sinapine into the cricket tissue was observed by target screening. Although this compound has antioxidative and anti-inflammatory properties, its bitterness can negatively affect the taste of the final product. On the other hand, antinutritious glucosinolate goitrin was not confirmed in any of the tested samples. In addition, markers of soya (daidzein, genistein, saponins) were observed in cricket tissue.This study comprehensively describes changes in cricket metabolome after modifying the feed composition with rapeseed meal as a by-product of rapeseed oil production. The results can contribute to the optimization of edible insect breeding to produce safe and quality products and to contribute to the functioning of the circular economy and the processing of by-products of food production.

Název v anglickém jazyce

Critical assessment of Jamaican field cricket metabolome after rapeseed meal added to feed

Popis výsledku anglicky

Constantly growing world population puts enormous pressure on food systems. One of the solutions how to supply the population with enough nutrients and especially proteins in the future is the production of edible insects. The main advantages of edible insects are high conversion of feed, small environmental footprint, and small demands on space and water. These advantages make edible insects a possible challenging sustainable alternative to traditional animal protein sources and make it a possible future common ingredient of food and feed. This potential usage of insects makes this commodity a growing concept and draws the attention of researchers who mainly focus on the impact of rearing conditions such as temperature on biomass gain, feed conversion, and nutritional properties. However, the effects of feed composition on the quality of edible insects are still quite unexplored, especially in terms of bioactive compounds and micronutrients profiles. Complementing knowledge in this field is particularly desirable for the future production of quality and safe edible insects.In this study, the effect of using by-product from rapeseed oil production (rapeseed meal) on metabolome of Jamaican field cricket (Gryllus assimilis) was investigated. Rapeseed meal was used in this experiment as a substitution for protein component (soya) in feed. Five groups of crickets differing in feed composition were analyzed. Attention was paid especially to the transfer of bioactive compounds. For the evaluation of the changes in cricket biomass, the metabolomic analysis using ultra-performance liquid chromatography coupled with high-resolution tandem mass spectrometry(U-HPLC-HRMS/MS) with subsequent use of chemometric tools (PCA, PLS-DA) was performed. Target screening of selected bioactive compounds such as sinapine or antinutritious glucosinolate goitrin was also performed.It was possible to distinguish the groups of Jamaican field cricket according to the feed composition and possible markers of rapeseed meal and soya in feed were identified. Transfer of sinapine into the cricket tissue was observed by target screening. Although this compound has antioxidative and anti-inflammatory properties, its bitterness can negatively affect the taste of the final product. On the other hand, antinutritious glucosinolate goitrin was not confirmed in any of the tested samples. In addition, markers of soya (daidzein, genistein, saponins) were observed in cricket tissue.This study comprehensively describes changes in cricket metabolome after modifying the feed composition with rapeseed meal as a by-product of rapeseed oil production. The results can contribute to the optimization of edible insect breeding to produce safe and quality products and to contribute to the functioning of the circular economy and the processing of by-products of food production.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/LM2018100" target="_blank" >LM2018100: Infrastruktura pro propagaci metrologie v potravinářství a výživě v České republice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů