Changes in edible insect metabolome after altering the feed composition by rapeseed meal

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F23%3A43926597" target="_blank" >RIV/60461373:22330/23:43926597 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Changes in edible insect metabolome after altering the feed composition by rapeseed meal

Popis výsledku v původním jazyce

Edible insects are promising novel food, which can serve as a sustainable source of animal proteins. Four species of edible insects are already authorized in the EU for human consumption and other species are in the process of authorization. The growing popularity of this concept demands ensuring the safety and quality of insect-based foods introduced on the market. Especially the effects of feed composition on the metabolome of edible insects are still rather unexplored. Expanding the knowledge on this topic is essential for the production of safe edible insects in the future. In this study, the effect of rapeseed meal, by-product from oil production, on metabolome of Jamaican field cricket (Gryllus assimilis) was investigated. The rapeseed meal was used as a substitution for soya, representing the protein component of the feed. UHPLC-HRMS/MS was applied for metabolomic analysis of 5 groups of crickets with the increasing rapeseed meal content in the feed. Using principal component analysis (PCA) and partial least squares discrimination analysis (PLS-DA), the samples were separated according to the feed composition. In combination with correlation analysis, possible markers of feed alteration in cricket biomass were found and identified based on the exact mass, isotopic profile, and fragmentation pattern of the respective ion. As expected, sinapine was identified as a marker of rapeseed. Although this compound might not be harmful, its bitterness can negatively affect the taste of the final product. Samples were also screened for the presence of antinutritive glucosinolate goitrin. This compound was not detected in any of the tested samples and, therefore, the transfer to the cricket tissues was not confirmed. In addition, the transfer of secondary metabolites typically occurring in soya (daidzein, genistein, saponins) was observed. The results can contribute to the optimization of edible insects farming and to the food industry’s circular economy functioning.

Název v anglickém jazyce

Changes in edible insect metabolome after altering the feed composition by rapeseed meal

Popis výsledku anglicky

Edible insects are promising novel food, which can serve as a sustainable source of animal proteins. Four species of edible insects are already authorized in the EU for human consumption and other species are in the process of authorization. The growing popularity of this concept demands ensuring the safety and quality of insect-based foods introduced on the market. Especially the effects of feed composition on the metabolome of edible insects are still rather unexplored. Expanding the knowledge on this topic is essential for the production of safe edible insects in the future. In this study, the effect of rapeseed meal, by-product from oil production, on metabolome of Jamaican field cricket (Gryllus assimilis) was investigated. The rapeseed meal was used as a substitution for soya, representing the protein component of the feed. UHPLC-HRMS/MS was applied for metabolomic analysis of 5 groups of crickets with the increasing rapeseed meal content in the feed. Using principal component analysis (PCA) and partial least squares discrimination analysis (PLS-DA), the samples were separated according to the feed composition. In combination with correlation analysis, possible markers of feed alteration in cricket biomass were found and identified based on the exact mass, isotopic profile, and fragmentation pattern of the respective ion. As expected, sinapine was identified as a marker of rapeseed. Although this compound might not be harmful, its bitterness can negatively affect the taste of the final product. Samples were also screened for the presence of antinutritive glucosinolate goitrin. This compound was not detected in any of the tested samples and, therefore, the transfer to the cricket tissues was not confirmed. In addition, the transfer of secondary metabolites typically occurring in soya (daidzein, genistein, saponins) was observed. The results can contribute to the optimization of edible insects farming and to the food industry’s circular economy functioning.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů