Analytical strategy for identification of unknown transformation products of mycotoxins after their decontamination by pulsed electric field technology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925448" target="_blank" >RIV/60461373:22330/22:43925448 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analytical strategy for identification of unknown transformation products of mycotoxins after their decontamination by pulsed electric field technology

Popis výsledku v původním jazyce

Mycotoxin contamination of cereals is a problem of a global scope. Different decontamination technologies are in use today, including the pulsed electric field (PEF). Our recent research on this topic revealed significant influence of PEF on decontamination of mycotoxins present in cereals. The overall reduction of trichothecenes, zearalenone, enniatins, beauvericin, and tentoxin in barley was up to 31, 48, 84, 36 and 46%, respectively. As we found out, besides the increased extractability into the electrolyte (water), the major reason of this reduction was mycotoxins’ degradation and/or transformation into the different reaction products. As the identification and characterization of these unknown reaction products is of a major importance in terms of follow up toxicity and risk assessment, we developed an analytical strategy enabling characterization and confirmation of these products by using the tools of advanced analytical chemistry, including the in silico modelling and high resolution mass spectrometry (HRMS). For creating the database of predicted reaction products, Zeneth software was used, and these compounds were further pre-screened by ultra-high performance liquid chromatography coupled with Q-orbitrap HRMS. Positive hints of this prescreening were further verified by evaluation of their retention times, and compliance of their HRMS/MS spectra with the in silico predictions. The determined degradation / transformation products of mycotoxins were mostly products of hydrolysis, elimination and/or oxidation.

Název v anglickém jazyce

Analytical strategy for identification of unknown transformation products of mycotoxins after their decontamination by pulsed electric field technology

Popis výsledku anglicky

Mycotoxin contamination of cereals is a problem of a global scope. Different decontamination technologies are in use today, including the pulsed electric field (PEF). Our recent research on this topic revealed significant influence of PEF on decontamination of mycotoxins present in cereals. The overall reduction of trichothecenes, zearalenone, enniatins, beauvericin, and tentoxin in barley was up to 31, 48, 84, 36 and 46%, respectively. As we found out, besides the increased extractability into the electrolyte (water), the major reason of this reduction was mycotoxins’ degradation and/or transformation into the different reaction products. As the identification and characterization of these unknown reaction products is of a major importance in terms of follow up toxicity and risk assessment, we developed an analytical strategy enabling characterization and confirmation of these products by using the tools of advanced analytical chemistry, including the in silico modelling and high resolution mass spectrometry (HRMS). For creating the database of predicted reaction products, Zeneth software was used, and these compounds were further pre-screened by ultra-high performance liquid chromatography coupled with Q-orbitrap HRMS. Positive hints of this prescreening were further verified by evaluation of their retention times, and compliance of their HRMS/MS spectra with the in silico predictions. The determined degradation / transformation products of mycotoxins were mostly products of hydrolysis, elimination and/or oxidation.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LM2018100" target="_blank" >LM2018100: Infrastruktura pro propagaci metrologie v potravinářství a výživě v České republice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů