THE IMPACT OF GROWTH STAGE AND GEOGRAPHICAL ORIGIN ON IN-VITRO ENZYME INHIBITORY EFFECT AND PHENOLIC PROFILE OF AGRIMONIA EUPATORIA EXTRACTS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F23%3A43928151" target="_blank" >RIV/60461373:22330/23:43928151 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

THE IMPACT OF GROWTH STAGE AND GEOGRAPHICAL ORIGIN ON IN-VITRO ENZYME INHIBITORY EFFECT AND PHENOLIC PROFILE OF AGRIMONIA EUPATORIA EXTRACTS

Popis výsledku v původním jazyce

Agrimonia eupatoria (Rosaceae), commonly known as agrimony, is a plant widely spread in the northern hemisphere that has been used in traditional medicine due to its antioxidant, anti-inflammatory, analgesic and hypotensive properties, amongst others. There is both in vitro and in vivo evidence [1] that the aforementioned health beneficial properties are related to agrimony high abundance in phenolic compounds. Although published studies have provided some insight on agrimony bioactivity and its phenolic profile, still there is not much information on how these parameters may be impacted by the plant growth stage as well as its geographical origin. To this end, in this study, we applied a multidisciplinary approach to analyse aqueous and ethanolic extracts originating by agrimony plants in four different growth stages (vegetative stage, beginning of flowering, full bloom and senescence) and cultivated in two different geographical locations in the Czech Republic (Hlohovec and Milovice). In total 30 samples were tested and their inhibitory effect against enzymes with important biochemical functions was monitored, namely acetylcholinesterase (AChE, EC 3.1.1.7), pancreatic lipase (PNLIP, EC 3.1.1.5) and tyrosinase (TYR, EC 1.14.18.1). Worthy to mention is that all enzyme assays were developed in-house providing high-throughput and cost-efficiency [2]. Indicatively, the TYR assay optimisation will be presented to demonstrate the analytical challenges faced in order to deliver robust and accurate results. Based on the preliminary results, the highest AChE inhibitory effect was monitored in the senescence (98% ± 2.5%, extract concentration 100 mg mL-1) whilst in the case of PNLIP at the beginning of flowering (74% ± 3.2%, extract concentration 100 mg mL-1). This indicates that maybe phenolic compounds expressed in different growth stages inhibited the enzyme active site in each case. Following the performed enzyme assays, a suspect screening metabolomic workflow was performed using an ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS) method. The attained polyphenol profile varied depending on the growth stage, with the most significant differences to be noticed in the senescence stage. Interestingly, differences were noticed for specific compounds for plants originating from a different location. All in all, the presented approach combines in vitro bioactivity measurements to high-end metabolomics delivering a dynamic profile of the phenolic content and its enzyme inhibitory potency.

Název v anglickém jazyce

THE IMPACT OF GROWTH STAGE AND GEOGRAPHICAL ORIGIN ON IN-VITRO ENZYME INHIBITORY EFFECT AND PHENOLIC PROFILE OF AGRIMONIA EUPATORIA EXTRACTS

Popis výsledku anglicky

Agrimonia eupatoria (Rosaceae), commonly known as agrimony, is a plant widely spread in the northern hemisphere that has been used in traditional medicine due to its antioxidant, anti-inflammatory, analgesic and hypotensive properties, amongst others. There is both in vitro and in vivo evidence [1] that the aforementioned health beneficial properties are related to agrimony high abundance in phenolic compounds. Although published studies have provided some insight on agrimony bioactivity and its phenolic profile, still there is not much information on how these parameters may be impacted by the plant growth stage as well as its geographical origin. To this end, in this study, we applied a multidisciplinary approach to analyse aqueous and ethanolic extracts originating by agrimony plants in four different growth stages (vegetative stage, beginning of flowering, full bloom and senescence) and cultivated in two different geographical locations in the Czech Republic (Hlohovec and Milovice). In total 30 samples were tested and their inhibitory effect against enzymes with important biochemical functions was monitored, namely acetylcholinesterase (AChE, EC 3.1.1.7), pancreatic lipase (PNLIP, EC 3.1.1.5) and tyrosinase (TYR, EC 1.14.18.1). Worthy to mention is that all enzyme assays were developed in-house providing high-throughput and cost-efficiency [2]. Indicatively, the TYR assay optimisation will be presented to demonstrate the analytical challenges faced in order to deliver robust and accurate results. Based on the preliminary results, the highest AChE inhibitory effect was monitored in the senescence (98% ± 2.5%, extract concentration 100 mg mL-1) whilst in the case of PNLIP at the beginning of flowering (74% ± 3.2%, extract concentration 100 mg mL-1). This indicates that maybe phenolic compounds expressed in different growth stages inhibited the enzyme active site in each case. Following the performed enzyme assays, a suspect screening metabolomic workflow was performed using an ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS) method. The attained polyphenol profile varied depending on the growth stage, with the most significant differences to be noticed in the senescence stage. Interestingly, differences were noticed for specific compounds for plants originating from a different location. All in all, the presented approach combines in vitro bioactivity measurements to high-end metabolomics delivering a dynamic profile of the phenolic content and its enzyme inhibitory potency.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10611 - Plant sciences, botany

Projekt

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Návaznosti

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Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů