Short- and medium-chain chlorinated paraffins in insect-based foodstuff

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F24%3A43930632" target="_blank" >RIV/60461373:22330/24:43930632 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Short- and medium-chain chlorinated paraffins in insect-based foodstuff

Popis výsledku v původním jazyce

Short- and medium-chain chlorinated paraffins (SCCPs and MCCPs, respectively) are emerging and ubiquitous groups of environmental pollutants associated with adverse effects on human health (such as endocrine disruption and possible carcinogenicity of SCCPs). They are classified based on their carbon chain lengths - SCCPs having C10 C13 and MCCPs having C14-C17). The analytical chemistry of CPs is a very challenging task, mainly due to the nature of the compounds (their technical mixtures contain thousands of isomers and homologues, which are practically inseparable by conventional chromatographic techniques) and the lack of suitable commercially available internal standards.In this study, risk assessment of SCCPs and MCCPs in a novel food category – edible insects – was evaluated. CPs were extracted from insect-based food from the Czech market (n=67) and samples obtained directly from farms (n=22) by ethyl acetate partitioning. The CPs were then isolated from co-extracting lipids using solid phase extraction on a silica gel (deactivated by 2% of water) and then analysed by gas chromatography coupled to high-resolution mass spectrometry operated in negative chemical ionisation (GC-NCI-HRMS; Agilent 7890B GC with coupled with Agilent 7200B quadrupole-time of flight mass spectrometer - GC/Q-TOF system; the CPs were separated on HP-5MS UI column, 15 m × 0.25 mm × 0.25 µm, all Agilent Technologies, USA). The method was validated, with recoveries in the range of 70 – 100% and repeatabilities (expressed as relative standard deviations) &lt;17%. The limits of quantification (LOQs) were 0.01 and 0.03 µg/g lipids (SCCPs and MCCPs, respectively).The CP concentrations in samples from the Czech market were in the range of &lt;0.01 – 21.36 (median 0.08) µg/g lipids and &lt;0.03 – 7.74 (median 0.09) µg/g lipids (SCCPs and MCCPs, respectively). The consumption of analysed samples would not lead to exceeding the tolerable daily intakes (30 and 4 µg/kg body weight, SCCPs and MCCPs, respectively). Nevertheless, the CPs dietary exposure is expected to be low in the Czech Republic, therefore the consumption of insect-based foodstuff would be a significant source of exposure to CPs, with possible increase of the share in the total dietary intake of insect-based food, due to the approval of edible insects being safe for human consumption in the EU.To find the possible source of contamination in the edible insects, samples obtained directly from farms were also analysed. The CP concentrations were in the range of &lt;0.09 – 2.16 (median 0.24) µg/g lipids and &lt;0.05 – 1.81 (median 0.44) µg/g lipids (SCCPs and MCCPs, respectively). The samples were more contaminated than samples from the market (by medians), with lower extremes.

Název v anglickém jazyce

Short- and medium-chain chlorinated paraffins in insect-based foodstuff

Popis výsledku anglicky

Short- and medium-chain chlorinated paraffins (SCCPs and MCCPs, respectively) are emerging and ubiquitous groups of environmental pollutants associated with adverse effects on human health (such as endocrine disruption and possible carcinogenicity of SCCPs). They are classified based on their carbon chain lengths - SCCPs having C10 C13 and MCCPs having C14-C17). The analytical chemistry of CPs is a very challenging task, mainly due to the nature of the compounds (their technical mixtures contain thousands of isomers and homologues, which are practically inseparable by conventional chromatographic techniques) and the lack of suitable commercially available internal standards.In this study, risk assessment of SCCPs and MCCPs in a novel food category – edible insects – was evaluated. CPs were extracted from insect-based food from the Czech market (n=67) and samples obtained directly from farms (n=22) by ethyl acetate partitioning. The CPs were then isolated from co-extracting lipids using solid phase extraction on a silica gel (deactivated by 2% of water) and then analysed by gas chromatography coupled to high-resolution mass spectrometry operated in negative chemical ionisation (GC-NCI-HRMS; Agilent 7890B GC with coupled with Agilent 7200B quadrupole-time of flight mass spectrometer - GC/Q-TOF system; the CPs were separated on HP-5MS UI column, 15 m × 0.25 mm × 0.25 µm, all Agilent Technologies, USA). The method was validated, with recoveries in the range of 70 – 100% and repeatabilities (expressed as relative standard deviations) &lt;17%. The limits of quantification (LOQs) were 0.01 and 0.03 µg/g lipids (SCCPs and MCCPs, respectively).The CP concentrations in samples from the Czech market were in the range of &lt;0.01 – 21.36 (median 0.08) µg/g lipids and &lt;0.03 – 7.74 (median 0.09) µg/g lipids (SCCPs and MCCPs, respectively). The consumption of analysed samples would not lead to exceeding the tolerable daily intakes (30 and 4 µg/kg body weight, SCCPs and MCCPs, respectively). Nevertheless, the CPs dietary exposure is expected to be low in the Czech Republic, therefore the consumption of insect-based foodstuff would be a significant source of exposure to CPs, with possible increase of the share in the total dietary intake of insect-based food, due to the approval of edible insects being safe for human consumption in the EU.To find the possible source of contamination in the edible insects, samples obtained directly from farms were also analysed. The CP concentrations were in the range of &lt;0.09 – 2.16 (median 0.24) µg/g lipids and &lt;0.05 – 1.81 (median 0.44) µg/g lipids (SCCPs and MCCPs, respectively). The samples were more contaminated than samples from the market (by medians), with lower extremes.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

30304 - Public and environmental health

Projekt

<a href="/cs/project/QK23020101" target="_blank" >QK23020101: Komplexní laboratorní strategie pro identifikaci druhů hmyzu určeného k lidské spotřebě a produkci zpracované živočišné bílkoviny, autentikace potravin na jeho bázi</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů