Effect of Mitophagy-Related Gene Regulation on Antioxidant Activity of Lager Yeast

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F24%3A43930691" target="_blank" >RIV/60461373:22330/24:43930691 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2306-5710/10/4/112" target="_blank" >https://www.mdpi.com/2306-5710/10/4/112</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/beverages10040112" target="_blank" >10.3390/beverages10040112</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of Mitophagy-Related Gene Regulation on Antioxidant Activity of Lager Yeast

Popis výsledku v původním jazyce

Lager yeast, which is mainly used for lager beer brewing, withstands significant oxidative stress during brewing process, leading to its rapid aging in serial beer brewing. Mitophagy plays a critical role in the antioxidant stress response of yeast. However, the relationship between mitophagy and the antioxidant capacity of yeast is still unclear. Previous studies indicated that ATG gene family in mitophagy significantly affects the antioxidant capacity of yeast cells in beer brewing. Herein, the expression of ATG8, ATG11, ATG32, DNM1, and MMM1 genes was regulated. The results showed that the overexpression of ATG8 and ATG11 significantly reduced the intracellular ROS contents to 52.05% and 22.57% of the initial state, respectively, and helped to maintain a high mitochondrial vitality during serial fermentation. Disruption of ATG8, ATG11 and ATG32 resulted in significant decrease in cell vitality when exposed to H2O2 stimulation. Meanwhile, the disruptions of these genes were detrimental to the balance of intracellular ROS. Excess DNM1 activity could affect the cellular energy balance and ATP depletion under prolonged stress conditions. The repression of MMM1 led to lower ATP levels during serial beer fermentation. The ATG8, ATG11, and ATG32 genes might be potential targets for regulating the antioxidant capacity of yeast. The current work provides new insights into improving the antioxidant capacity of yeast through mitophagy regulation.

Název v anglickém jazyce

Effect of Mitophagy-Related Gene Regulation on Antioxidant Activity of Lager Yeast

Popis výsledku anglicky

Lager yeast, which is mainly used for lager beer brewing, withstands significant oxidative stress during brewing process, leading to its rapid aging in serial beer brewing. Mitophagy plays a critical role in the antioxidant stress response of yeast. However, the relationship between mitophagy and the antioxidant capacity of yeast is still unclear. Previous studies indicated that ATG gene family in mitophagy significantly affects the antioxidant capacity of yeast cells in beer brewing. Herein, the expression of ATG8, ATG11, ATG32, DNM1, and MMM1 genes was regulated. The results showed that the overexpression of ATG8 and ATG11 significantly reduced the intracellular ROS contents to 52.05% and 22.57% of the initial state, respectively, and helped to maintain a high mitochondrial vitality during serial fermentation. Disruption of ATG8, ATG11 and ATG32 resulted in significant decrease in cell vitality when exposed to H2O2 stimulation. Meanwhile, the disruptions of these genes were detrimental to the balance of intracellular ROS. Excess DNM1 activity could affect the cellular energy balance and ATP depletion under prolonged stress conditions. The repression of MMM1 led to lower ATP levels during serial beer fermentation. The ATG8, ATG11, and ATG32 genes might be potential targets for regulating the antioxidant capacity of yeast. The current work provides new insights into improving the antioxidant capacity of yeast through mitophagy regulation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Beverages

ISSN

2306-5710

e-ISSN

2306-5710

Svazek periodika

10

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

18

Strana od-do

—

Kód UT WoS článku

001386830900001

EID výsledku v databázi Scopus

2-s2.0-85213576055