Assessment and authentication of edible insects available at the market

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F24%3A43930698" target="_blank" >RIV/60461373:22330/24:43930698 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Assessment and authentication of edible insects available at the market

Popis výsledku v původním jazyce

The European market with edible insects and insect-based products is continuously growing, until now, four species have been authorised as a novel food: Tenebrio molitor, Acheta domesticus, Locusta migratoria and Alphitobius diaperinus (Regulation (EU) 2015/2283). Alike in case of other high nutritional value commodities, fraudulent practices cannot be avoided, therefore effective tools are needed for label control and authentication. The research mainly focused on untargeted metabolomic analysis and targeted screening of polar metabolites in available insect samples. In total, extracts (aqueous ethanol 1:1, v/v) prepared from 36 authentic insect samples (9 species, including authorized species in the EU, from nine different Czech breeders) and 67 samples of different commercial edible insect-based products were subjected to analysis performed by ultra-high performance liquid chromatography coupled with high resolution tandem mass spectrometry (UHPLC-HRMS/MS). Multivariate analysis (PCA, PLS-DA) revealed differences in metabolome of tested insect species. The insect-based products clustered with authentic insect samples in PCA and PLS-DA score plots. In total, 31 potential markers of 9 insect species were identified. For example, leucopterin, a compound present in the insect wings, as a marker of adult insects (e.g. Locusta migratoria and Schistocerca gregaria). The identified markers were incorporated into the in-house metabolomic marker database, which at present contains 51 potential markers, which their applicability was successfully verified on two sets of commercial samples. The results of this study have identified the challenges for follow-up entometabolomics research, especially for the authentication of edible insects and products thereof.

Název v anglickém jazyce

Assessment and authentication of edible insects available at the market

Popis výsledku anglicky

The European market with edible insects and insect-based products is continuously growing, until now, four species have been authorised as a novel food: Tenebrio molitor, Acheta domesticus, Locusta migratoria and Alphitobius diaperinus (Regulation (EU) 2015/2283). Alike in case of other high nutritional value commodities, fraudulent practices cannot be avoided, therefore effective tools are needed for label control and authentication. The research mainly focused on untargeted metabolomic analysis and targeted screening of polar metabolites in available insect samples. In total, extracts (aqueous ethanol 1:1, v/v) prepared from 36 authentic insect samples (9 species, including authorized species in the EU, from nine different Czech breeders) and 67 samples of different commercial edible insect-based products were subjected to analysis performed by ultra-high performance liquid chromatography coupled with high resolution tandem mass spectrometry (UHPLC-HRMS/MS). Multivariate analysis (PCA, PLS-DA) revealed differences in metabolome of tested insect species. The insect-based products clustered with authentic insect samples in PCA and PLS-DA score plots. In total, 31 potential markers of 9 insect species were identified. For example, leucopterin, a compound present in the insect wings, as a marker of adult insects (e.g. Locusta migratoria and Schistocerca gregaria). The identified markers were incorporated into the in-house metabolomic marker database, which at present contains 51 potential markers, which their applicability was successfully verified on two sets of commercial samples. The results of this study have identified the challenges for follow-up entometabolomics research, especially for the authentication of edible insects and products thereof.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

21101 - Food and beverages

Projekt

<a href="/cs/project/QK23020101" target="_blank" >QK23020101: Komplexní laboratorní strategie pro identifikaci druhů hmyzu určeného k lidské spotřebě a produkci zpracované živočišné bílkoviny, autentikace potravin na jeho bázi</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů