Entometabolomics of edible insect species reared under different conditions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F24%3A43930702" target="_blank" >RIV/60461373:22330/24:43930702 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Entometabolomics of edible insect species reared under different conditions

Popis výsledku v původním jazyce

Edible insects represent an appealing source of future food with the potential to cover the increasing global need for nutrients. In the EU, 4 species have been authorized as a novel food (Regulation (EU) 2015/2283). However, there are still many gaps in the availability of comprehensive laboratory strategies enabling further improvement of insect-based product quality, safety and/or authenticity assessment and bridging these attributes with rearing conditions. In the presented study, a total of 10 insect species (including Acheta domesticus, Tenebrio molitor, Locusta migratoria, and Alphitobius diaperinus) obtained from different local farmers were collected over the course of two years. These samples differed not only in rearing conditions but also in the way of killing. Non-target screening, entometabolomics, based on U-HPLC-HRMS/MS followed by multivariate analysis of generated data was employed to characterize both polar and non-polar extracts. Safe distinguishing of individual species was achieved, with polar extracts resulting in tighter clustering. Moreover, it was possible to identify the rearing sites of a given species, due to the association between metabolome and particular feeding conditions. Potential markers of species and killing methods were selected. For example, dopamine-3-O-sulfate, a compound associated with cuticle sclerotization, which was detected as a marker of insects in the adult development stage. The selected markers were used to establish the HRMS/MS spectral library which is essential for testing marker selectivity. In case of nonpolar extracts, lipidomic screening against MS-Dial lipids library illustrated the differences in lipid composition on feed (for example, lipids with bound fatty acid 20:5 were detected in cricket samples fed fish or insect meal). The possibility of enhancing the nutritional value of insects by feeding them bioactive compound-rich feed has been documented. Not only the increase /profile change of carotenoids, tocopherols, and essential fatty acids in the mealworm tissues was observed when their sources such as carrot, spinach, and sunflower seeds were added to the feed, but also the change in the entire metabolome and lipidome was recorded. The results of this study demonstrate the potential of entometabolomic analysis as a challenging tool complementing conventional other omics currently used in laboratories concerned with this ´new´ food commodity.

Název v anglickém jazyce

Entometabolomics of edible insect species reared under different conditions

Popis výsledku anglicky

Edible insects represent an appealing source of future food with the potential to cover the increasing global need for nutrients. In the EU, 4 species have been authorized as a novel food (Regulation (EU) 2015/2283). However, there are still many gaps in the availability of comprehensive laboratory strategies enabling further improvement of insect-based product quality, safety and/or authenticity assessment and bridging these attributes with rearing conditions. In the presented study, a total of 10 insect species (including Acheta domesticus, Tenebrio molitor, Locusta migratoria, and Alphitobius diaperinus) obtained from different local farmers were collected over the course of two years. These samples differed not only in rearing conditions but also in the way of killing. Non-target screening, entometabolomics, based on U-HPLC-HRMS/MS followed by multivariate analysis of generated data was employed to characterize both polar and non-polar extracts. Safe distinguishing of individual species was achieved, with polar extracts resulting in tighter clustering. Moreover, it was possible to identify the rearing sites of a given species, due to the association between metabolome and particular feeding conditions. Potential markers of species and killing methods were selected. For example, dopamine-3-O-sulfate, a compound associated with cuticle sclerotization, which was detected as a marker of insects in the adult development stage. The selected markers were used to establish the HRMS/MS spectral library which is essential for testing marker selectivity. In case of nonpolar extracts, lipidomic screening against MS-Dial lipids library illustrated the differences in lipid composition on feed (for example, lipids with bound fatty acid 20:5 were detected in cricket samples fed fish or insect meal). The possibility of enhancing the nutritional value of insects by feeding them bioactive compound-rich feed has been documented. Not only the increase /profile change of carotenoids, tocopherols, and essential fatty acids in the mealworm tissues was observed when their sources such as carrot, spinach, and sunflower seeds were added to the feed, but also the change in the entire metabolome and lipidome was recorded. The results of this study demonstrate the potential of entometabolomic analysis as a challenging tool complementing conventional other omics currently used in laboratories concerned with this ´new´ food commodity.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10616 - Entomology

Projekt

<a href="/cs/project/QK23020101" target="_blank" >QK23020101: Komplexní laboratorní strategie pro identifikaci druhů hmyzu určeného k lidské spotřebě a produkci zpracované živočišné bílkoviny, autentikace potravin na jeho bázi</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů