Determination of Selected Biologically Active Substances in Dietary Supplements and the Adaptogenic Herb Rhaponticum carthamoides Using HPLC-MS/MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F25%3A43931534" target="_blank" >RIV/60461373:22330/25:43931534 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s12161-025-02805-y" target="_blank" >https://link.springer.com/article/10.1007/s12161-025-02805-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12161-025-02805-y" target="_blank" >10.1007/s12161-025-02805-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of Selected Biologically Active Substances in Dietary Supplements and the Adaptogenic Herb Rhaponticum carthamoides Using HPLC-MS/MS

Popis výsledku v původním jazyce

Rhaponticum carthamoides (Willd.) Iljin, widely known as maral root, is an adaptogenic herb that has been used in traditional medicine for centuries. Several different classes of compounds were identified in R. carthamoides, including phytosteroids, flavonoids, and phenolic acids. Nowadays, R. carthamoides is available on the market in many forms, such as dried root, milled plant, capsules, and tinctures. This study presents the development and optimisation of four HPLC-MS/MS methods for the determination of 37 selected bioactive compounds in both plant and dietary supplements. Due to insufficient ionisation at the ion source, stigmasterol and ss-sitosterol required derivatization, which was developed and optimised using 2-fluoro-1-methylpyridinium p-toluenesulfonate. The dried plant samples were extracted with 60% ethanol or tert-butyl methyl ether. The results showed that chlorogenic acid and 20-hydroxyecdysone were the most abundant. To reduce the dimensionality of the obtained data, factor analysis was applied. Based on the distribution of selected compounds, four clusters were observed: tinctures, seeds, root, and shoot systems of the herb. Using the developed methods, selected bioactive compounds in the herb and dietary supplements were successfully quantified, and their distribution in different organs of R. carthamoides was clarified.

Název v anglickém jazyce

Determination of Selected Biologically Active Substances in Dietary Supplements and the Adaptogenic Herb Rhaponticum carthamoides Using HPLC-MS/MS

Popis výsledku anglicky

Rhaponticum carthamoides (Willd.) Iljin, widely known as maral root, is an adaptogenic herb that has been used in traditional medicine for centuries. Several different classes of compounds were identified in R. carthamoides, including phytosteroids, flavonoids, and phenolic acids. Nowadays, R. carthamoides is available on the market in many forms, such as dried root, milled plant, capsules, and tinctures. This study presents the development and optimisation of four HPLC-MS/MS methods for the determination of 37 selected bioactive compounds in both plant and dietary supplements. Due to insufficient ionisation at the ion source, stigmasterol and ss-sitosterol required derivatization, which was developed and optimised using 2-fluoro-1-methylpyridinium p-toluenesulfonate. The dried plant samples were extracted with 60% ethanol or tert-butyl methyl ether. The results showed that chlorogenic acid and 20-hydroxyecdysone were the most abundant. To reduce the dimensionality of the obtained data, factor analysis was applied. Based on the distribution of selected compounds, four clusters were observed: tinctures, seeds, root, and shoot systems of the herb. Using the developed methods, selected bioactive compounds in the herb and dietary supplements were successfully quantified, and their distribution in different organs of R. carthamoides was clarified.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40500 - Other agricultural sciences

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Analytical Methods

ISSN

1936-9751

e-ISSN

1936-976X

Svazek periodika

Neuveden

Číslo periodika v rámci svazku

05.05.2025

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

18

Strana od-do

nestránkováno

Kód UT WoS článku

001480971800001

EID výsledku v databázi Scopus

2-s2.0-105004035105