Quantification of copper and zinc species fractions in legume seeds extracts by SEC/ICP-MS: validation and uncertainty estimation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F02%3A00007187" target="_blank" >RIV/60461373:22340/02:00007187 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/02:00007187

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Quantification of copper and zinc species fractions in legume seeds extracts by SEC/ICP-MS: validation and uncertainty estimation

Popis výsledku v původním jazyce

Fractions of Cu and Zn species in legume samples (common white bean, pea, chick pea and lentil seeds and defatted soybean flour) were analyzed by on-line hyphenation of size exclusion chromatography and inductively coupled plasma-mass spectrometry. Samples were extracted by 0.02 mol/L Tris-HCl buffer solution, pH = 7.5. The extraction efficiency lay in the region 60 - 90 % and 60 - 80 % for Cu and Zn respectively. Quantification of elements in the individual chromatographic fractions was carried out byisotope dilution (ID) and external calibration (EC) techniques. For ID analysis the chromatographic effluent was mixed with the flow of 65Cu and 68Zn isotope enriched solution and the isotope ratio values 63Cu/65Cu and (64Zn+66Zn)/68Zn were measured. Inthe case of EC technique calibration solutions of elements were injected to the flow of mobile phase by the second injector. Prior entering detector the effluent was mixed with the flow of internal standard solution (In, 50 g/L). Both met

Název v anglickém jazyce

Quantification of copper and zinc species fractions in legume seeds extracts by SEC/ICP-MS: validation and uncertainty estimation

Popis výsledku anglicky

Fractions of Cu and Zn species in legume samples (common white bean, pea, chick pea and lentil seeds and defatted soybean flour) were analyzed by on-line hyphenation of size exclusion chromatography and inductively coupled plasma-mass spectrometry. Samples were extracted by 0.02 mol/L Tris-HCl buffer solution, pH = 7.5. The extraction efficiency lay in the region 60 - 90 % and 60 - 80 % for Cu and Zn respectively. Quantification of elements in the individual chromatographic fractions was carried out byisotope dilution (ID) and external calibration (EC) techniques. For ID analysis the chromatographic effluent was mixed with the flow of 65Cu and 68Zn isotope enriched solution and the isotope ratio values 63Cu/65Cu and (64Zn+66Zn)/68Zn were measured. Inthe case of EC technique calibration solutions of elements were injected to the flow of mobile phase by the second injector. Prior entering detector the effluent was mixed with the flow of internal standard solution (In, 50 g/L). Both met

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F00%2F0581" target="_blank" >GA525/00/0581: Speciační analýza stopových prvků v potravinách</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

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Svazek periodika

57

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

1133-1142

Kód UT WoS článku

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EID výsledku v databázi Scopus

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