Application of size exclusion chromatography - inductively coupled plasma - mass spectrometry for fractionation of element species in seeds of legumes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F02%3A00007174" target="_blank" >RIV/60461373:22340/02:00007174 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/02:00007174

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Application of size exclusion chromatography - inductively coupled plasma - mass spectrometry for fractionation of element species in seeds of legumes

Popis výsledku v původním jazyce

Fractionation of soluble species of P, Mn, Fe, Co, Ni, Cu, Zn, Se and Mo in pea and lentil seeds was made by on-line hyphenation of SEC and ICP-MS. Seed samples were extracted by 0.02 mol l-1 Tris-HCl buffer solution, pH=7.5. SEC was performed on Superdex 75 and Superdex Peptide columns (300 10 mm) with the same buffer solution as the mobile phase. Monitoring of oxide ion 47(PO)+ was used for detection of phosphorus compounds. Other elements were detected as ions of 55Mn, 57Fe, 59Co, 62Ni, 65Cu, 66Zn, 82Se and 95Mo nuclides. Elements in individual elution zones were quantified using external calibration. Complete chromatographic recoveries of elements were found in cases of phosphorus, nickel and copper. Substantial parts of manganese and zinc, as wellas traces of cobalt, selenium and molybdenum are retained on the column. Injection of EDTA solution removes these elements from the column. Chromatographic profiles of pea and lentil samples are very similar for all elements except Mo. Ma

Název v anglickém jazyce

Application of size exclusion chromatography - inductively coupled plasma - mass spectrometry for fractionation of element species in seeds of legumes

Popis výsledku anglicky

Fractionation of soluble species of P, Mn, Fe, Co, Ni, Cu, Zn, Se and Mo in pea and lentil seeds was made by on-line hyphenation of SEC and ICP-MS. Seed samples were extracted by 0.02 mol l-1 Tris-HCl buffer solution, pH=7.5. SEC was performed on Superdex 75 and Superdex Peptide columns (300 10 mm) with the same buffer solution as the mobile phase. Monitoring of oxide ion 47(PO)+ was used for detection of phosphorus compounds. Other elements were detected as ions of 55Mn, 57Fe, 59Co, 62Ni, 65Cu, 66Zn, 82Se and 95Mo nuclides. Elements in individual elution zones were quantified using external calibration. Complete chromatographic recoveries of elements were found in cases of phosphorus, nickel and copper. Substantial parts of manganese and zinc, as wellas traces of cobalt, selenium and molybdenum are retained on the column. Injection of EDTA solution removes these elements from the column. Chromatographic profiles of pea and lentil samples are very similar for all elements except Mo. Ma

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F00%2F0581" target="_blank" >GA525/00/0581: Speciační analýza stopových prvků v potravinách</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography B

ISSN

1570-0232

e-ISSN

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Svazek periodika

778

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

179-187

Kód UT WoS článku

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EID výsledku v databázi Scopus

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