Vývoj mikrofluidního čipu pro heterogenní imunoanalýzu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F06%3A00016651" target="_blank" >RIV/60461373:22340/06:00016651 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of a microfluidic chip for heterogeneous immunoassay

Popis výsledku v původním jazyce

This work is focused on development of an experimental microchip for heterogeneous immunoassay. Polystyrene substrate is used as the material for fabrication of the microfluidic chip. Techniques of hot embossing and micro-milling are employed in the fabrication. The central part of the chip contains immobilized protein molecules with biological affinity (Protein A - PA). Two procedures of PA immobilization have been studied: (i) passive sorption and (ii) covalent crosslinking of protein layers. It has been found that both these methods are proper. In the one-step assay, liquid samples containing the complementary molecules (human immunoglobulins FITC labeled - hIgG) are dosed in the place of the immobilized layer through the inlet/outlet microfluidic channels. A sandwich (multi-step) system Protein A - human immunoglobulins - goat immunoglobulins (FITC labeled - gIgG) is also studied. After washing out, the fluorescence intensity in microchannels is proportional to the amount of the fo

Název v anglickém jazyce

Development of a microfluidic chip for heterogeneous immunoassay

Popis výsledku anglicky

This work is focused on development of an experimental microchip for heterogeneous immunoassay. Polystyrene substrate is used as the material for fabrication of the microfluidic chip. Techniques of hot embossing and micro-milling are employed in the fabrication. The central part of the chip contains immobilized protein molecules with biological affinity (Protein A - PA). Two procedures of PA immobilization have been studied: (i) passive sorption and (ii) covalent crosslinking of protein layers. It has been found that both these methods are proper. In the one-step assay, liquid samples containing the complementary molecules (human immunoglobulins FITC labeled - hIgG) are dosed in the place of the immobilized layer through the inlet/outlet microfluidic channels. A sandwich (multi-step) system Protein A - human immunoglobulins - goat immunoglobulins (FITC labeled - gIgG) is also studied. After washing out, the fluorescence intensity in microchannels is proportional to the amount of the fo

Druh

D - Stať ve sborníku

CEP obor

CI - Průmyslová chemie a chemické inženýrství

OECD FORD obor

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Projekt

<a href="/cs/project/GP104%2F03%2FD006" target="_blank" >GP104/03/D006: Studium mikroreaktorů pro bioinženýrské aplikace</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Sborník 17th International Congress of Chemical and Process Engineering

ISBN

80-86059-45-6

ISSN

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e-ISSN

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Počet stran výsledku

7

Strana od-do

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Název nakladatele

Czech Society of Chemical Engineering

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

27. 8. 2006

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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