Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F13%3A43895409" target="_blank" >RIV/60461373:22340/13:43895409 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.fbp.2013.06.001" target="_blank" >http://dx.doi.org/10.1016/j.fbp.2013.06.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fbp.2013.06.001" target="_blank" >10.1016/j.fbp.2013.06.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase

Popis výsledku v původním jazyce

The effect of processing method on the properties of cross-linked chitosan microparticles and on the enzymatic activity of laccase immobilized in the particles has been investigated. Chitosan has been cross-linked by tri-polyphosphate (TPP) using two methods ? the so called ex situ cross-linking whereby the solutions of chitosan, TPP and the enzyme have been pre-mixed and spray-dried by a standard two-fluid kinetic nozzle, and a novel in situ cross-linking method, whereby the solutions have been contacted at the tip of a three-fluid nozzle and cross-linking occurred within a drying droplet. The influence of the cross-linking method on the particle size and morphology, surface charge, and swelling ratio has been determined. The enzymatic activity of laccase toward the oxidation of a chromophore substrate (ABTS) has been systematically investigated and found to be superior in particles produced by the in situ cross-linking method.

Název v anglickém jazyce

Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase

Popis výsledku anglicky

The effect of processing method on the properties of cross-linked chitosan microparticles and on the enzymatic activity of laccase immobilized in the particles has been investigated. Chitosan has been cross-linked by tri-polyphosphate (TPP) using two methods ? the so called ex situ cross-linking whereby the solutions of chitosan, TPP and the enzyme have been pre-mixed and spray-dried by a standard two-fluid kinetic nozzle, and a novel in situ cross-linking method, whereby the solutions have been contacted at the tip of a three-fluid nozzle and cross-linking occurred within a drying droplet. The influence of the cross-linking method on the particle size and morphology, surface charge, and swelling ratio has been determined. The enzymatic activity of laccase toward the oxidation of a chromophore substrate (ABTS) has been systematically investigated and found to be superior in particles produced by the in situ cross-linking method.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CI - Průmyslová chemie a chemické inženýrství

OECD FORD obor

—

Projekt

<a href="/cs/project/MEB061108" target="_blank" >MEB061108: Vývoj antibakteriálních polymerů na chitosanové bázi pro biomedicínské aplikace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food and Bioproducts Processing

ISSN

0960-3085

e-ISSN

—

Svazek periodika

91

Číslo periodika v rámci svazku

C4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

14

Strana od-do

525-533

Kód UT WoS článku

000328009100027

EID výsledku v databázi Scopus

—