Determination of the maximum operating range of hydrodynamic stress in mammalian cell culture

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F15%3A43900188" target="_blank" >RIV/60461373:22340/15:43900188 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jbiotec.2014.12.003" target="_blank" >http://dx.doi.org/10.1016/j.jbiotec.2014.12.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jbiotec.2014.12.003" target="_blank" >10.1016/j.jbiotec.2014.12.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of the maximum operating range of hydrodynamic stress in mammalian cell culture

Popis výsledku v původním jazyce

Application of quality by design (QbD) requires identification of the maximum operating range for parameters affecting the cell culture process. These include hydrodynamic stress, mass transfer or gradients in dissolved oxygen and pH. Since most of theseare affected by the impeller design and speed, the main goal of this work was to identify a maximum operating range for hydrodynamic stress, where no variation of cell growth, productivity and product quality can be ensured. Two scale-down models were developed operating under laminar and turbulent condition, generating repetitive oscillating hydrodynamic stress with maximum stress values ranging from 0.4 to 420 Pa, to compare the effect of the different flow regimes on the cells behavior. Two manufacturing cell lines (CHO and Sp2/0) used for the synthesis of therapeutic proteins were employed in this study. For both cell lines multiple process outputs were used to determine the threshold values of hydrodynamic stress, such as cell gro

Název v anglickém jazyce

Determination of the maximum operating range of hydrodynamic stress in mammalian cell culture

Popis výsledku anglicky

Application of quality by design (QbD) requires identification of the maximum operating range for parameters affecting the cell culture process. These include hydrodynamic stress, mass transfer or gradients in dissolved oxygen and pH. Since most of theseare affected by the impeller design and speed, the main goal of this work was to identify a maximum operating range for hydrodynamic stress, where no variation of cell growth, productivity and product quality can be ensured. Two scale-down models were developed operating under laminar and turbulent condition, generating repetitive oscillating hydrodynamic stress with maximum stress values ranging from 0.4 to 420 Pa, to compare the effect of the different flow regimes on the cells behavior. Two manufacturing cell lines (CHO and Sp2/0) used for the synthesis of therapeutic proteins were employed in this study. For both cell lines multiple process outputs were used to determine the threshold values of hydrodynamic stress, such as cell gro

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CI - Průmyslová chemie a chemické inženýrství

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF BIOTECHNOLOGY

ISSN

0168-1656

e-ISSN

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Svazek periodika

194

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

100-109

Kód UT WoS článku

000347721700014

EID výsledku v databázi Scopus

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