Characterization of hydrodynamic stress in ambr250 (R) bioreactor system and its impact on mammalian cell culture

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F22%3A43925005" target="_blank" >RIV/60461373:22340/22:43925005 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1369703X21003168?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1369703X21003168?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bej.2021.108240" target="_blank" >10.1016/j.bej.2021.108240</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of hydrodynamic stress in ambr250 (R) bioreactor system and its impact on mammalian cell culture

Popis výsledku v původním jazyce

During the cell culture in stirred aerated vessels, both stirring and sparging is required for proper homogenization of a mixture and oxygen delivery. Quality by design (QbD) in biopharmaceutical industry aims at identification of the impact of mass transfer, dissolved oxygen, pH and hydrodynamic stress on the cell behavior, productivity and product quality. Most of these properties are influenced by impeller motion and sparger aeration. In this research, the shear sensitive poly(methyl methacrylate) (PMMA) aggregates are used as probes capable of determining the maximum hydrodynamic stress (tau(max)) present in the ambr250 (R) bioreactor. The results from our micro-aggregate probes are connected with the cultivation data using two Chinese hamster ovary K1 glutamine synthetase mediated cell lines (CHO-K1 GS) to determine the stress threshold, below which no significant impact of operating conditions on cell behavior is observed. Such calibrated system can be in the future used as a scale-down model of large-scale bioreactors to test cell robustness during process development.

Název v anglickém jazyce

Characterization of hydrodynamic stress in ambr250 (R) bioreactor system and its impact on mammalian cell culture

Popis výsledku anglicky

During the cell culture in stirred aerated vessels, both stirring and sparging is required for proper homogenization of a mixture and oxygen delivery. Quality by design (QbD) in biopharmaceutical industry aims at identification of the impact of mass transfer, dissolved oxygen, pH and hydrodynamic stress on the cell behavior, productivity and product quality. Most of these properties are influenced by impeller motion and sparger aeration. In this research, the shear sensitive poly(methyl methacrylate) (PMMA) aggregates are used as probes capable of determining the maximum hydrodynamic stress (tau(max)) present in the ambr250 (R) bioreactor. The results from our micro-aggregate probes are connected with the cultivation data using two Chinese hamster ovary K1 glutamine synthetase mediated cell lines (CHO-K1 GS) to determine the stress threshold, below which no significant impact of operating conditions on cell behavior is observed. Such calibrated system can be in the future used as a scale-down model of large-scale bioreactors to test cell robustness during process development.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20401 - Chemical engineering (plants, products)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochemical Engineering Journal

ISSN

1369-703X

e-ISSN

1873-295X

Svazek periodika

177

Číslo periodika v rámci svazku

JAN 2022

Stát vydavatele periodika

BE - Belgické království

Počet stran výsledku

9

Strana od-do

108240

Kód UT WoS článku

000717092700010

EID výsledku v databázi Scopus

2-s2.0-85118537595